METHODOLOGY DEVELOPMENT FOR THE ELUCIDATION OF PHOSPHORYLATION SITES ON PROTEIN

阐明蛋白质磷酸化位点的方法学开发

• 批准号: 7355077
• 负责人: Andrew N. Krutchinsky
• 金额: $ 0.62万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7355077
• 关键词: METHODOLOGY; DEVELOPMENT; ELUCIDATION; PHOSPHORYLATION; SITES

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. we designed a PCR template for homologous replacement of the endogenous protein promoters in yeast to inducible promoters. Expressed several protein substrates of endogenous kinases from these inducible promoters and in order to map phosphorylation sites on the proteins. We are testing the technique by mapping phosphorylation sites on several of these proteins including mcm3, bud3, boi2, lte1, ned1.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。我们设计了一个PCR模板，用于将酵母中的内源蛋白启动子同源替换为诱导型启动子。从这些诱导型启动子表达内源性激酶的几种蛋白质底物，以定位蛋白质上的磷酸化位点。我们正在通过绘制包括mcm 3，bud 3，boi 2，lte 1，ned 1在内的几种蛋白质的磷酸化位点来测试这项技术。