METHODOLOGY DEVELOPMENT FOR THE ELUCIDATION OF PHOSPHORYLATION SITES ON PROTEIN
阐明蛋白质磷酸化位点的方法学开发
基本信息
- 批准号:7722205
- 负责人:
- 金额:$ 0.22万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2008
- 资助国家:美国
- 起止时间:2008-03-01 至 2009-02-28
- 项目状态:已结题
- 来源:
- 关键词:Computer Retrieval of Information on Scientific Projects DatabaseDevelopmentFundingGrantInstitutionMapsMethodologyPhosphorylation SitePhosphotransferasesPolymerase Chain ReactionProteinsResearchResearch PersonnelResourcesSourceTechniquesTestingUnited States National Institutes of HealthYeastsdesignpromoter
项目摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
we designed a PCR template for homologous replacement of the endogenous protein promoters in yeast to inducible promoters. Expressed several protein substrates of endogenous kinases from these inducible promoters and in order to map phosphorylation sites on the proteins. We are testing the technique by mapping phosphorylation sites on several of these proteins including mcm3, bud3, boi2, lte1, ned1.
该子项目是利用该技术的众多研究子项目之一
资源由 NIH/NCRR 资助的中心拨款提供。子项目及
研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金,
因此可以在其他 CRISP 条目中表示。列出的机构是
对于中心来说,它不一定是研究者的机构。
我们设计了一个 PCR 模板,用于将酵母中的内源蛋白启动子同源替换为诱导型启动子。从这些诱导型启动子表达内源激酶的几种蛋白质底物,以便绘制蛋白质上的磷酸化位点图。我们正在通过绘制其中几种蛋白质(包括 mcm3、bud3、boi2、lte1、ned1)上的磷酸化位点来测试该技术。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Andrew N. Krutchinsky其他文献
High-capacity ion trap coupled to a time-of-flight mass spectrometer for comprehensive linked scans with no scanning losses
- DOI:
10.1016/j.ijms.2010.09.013 - 发表时间:
2011-03-30 - 期刊:
- 影响因子:
- 作者:
Sunnie Myung;Herbert Cohen;David Fenyö;Julio C. Padovan;Andrew N. Krutchinsky;Brian T. Chait - 通讯作者:
Brian T. Chait
Andrew N. Krutchinsky的其他文献
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{{ truncateString('Andrew N. Krutchinsky', 18)}}的其他基金
A NOVEL HIGH-CAPACITY ION TRAP-QUADRUPOLE TANDEM MASS SPECTROMETER
新型大容量离子阱四极杆串联质谱仪
- 批准号:
7954054 - 财政年份:2009
- 资助金额:
$ 0.22万 - 项目类别:
DESIGN/CONSTRUCT/TEST A NEW MASS SPECTROM FOR PROFILING PHOSPHORYLATION SITES
设计/构建/测试用于分析磷酸化位点的新质谱
- 批准号:
7954068 - 财政年份:2009
- 资助金额:
$ 0.22万 - 项目类别:
A NOVEL HIGH-CAPACITY ION TRAP-QUADRUPOLE TANDEM MASS SPECTROMETER
新型大容量离子阱四极杆串联质谱仪
- 批准号:
7722188 - 财政年份:2008
- 资助金额:
$ 0.22万 - 项目类别:
DESIGN/CONSTRUCT/TEST A NEW MASS SPECTROM FOR PROFILING PHOSPHORYLATION SITES
设计/构建/测试用于分析磷酸化位点的新质谱
- 批准号:
7722204 - 财政年份:2008
- 资助金额:
$ 0.22万 - 项目类别:
DESIGN/CONSTRUCT/TEST A NEW MASS SPECTROM FOR PROFILING PHOSPHORYLATION SITES
设计/构建/测试用于分析磷酸化位点的新质谱
- 批准号:
7355076 - 财政年份:2006
- 资助金额:
$ 0.22万 - 项目类别:
DESIGN & DEVELOPMENT OF NEW MALDI ION TRAP MASS SPECTROMETERS
设计
- 批准号:
7355048 - 财政年份:2006
- 资助金额:
$ 0.22万 - 项目类别:
IMPROVED METHODS FOR THE ANALYSIS OF PROTEIN COMPLEXES
蛋白质复合物分析方法的改进
- 批准号:
7355078 - 财政年份:2006
- 资助金额:
$ 0.22万 - 项目类别:
METHODOLOGY DEVELOPMENT FOR THE ELUCIDATION OF PHOSPHORYLATION SITES ON PROTEIN
阐明蛋白质磷酸化位点的方法学开发
- 批准号:
7355077 - 财政年份:2006
- 资助金额:
$ 0.22万 - 项目类别:
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