Design of tissue engineering bioreactors using Magnetic resonance imaging and two photon microscopy

使用磁共振成像和双光子显微镜设计组织工程生物反应器

• 批准号: EP/C535405/1
• 负责人: Matthew Lim
• 金额: $ 17.9万
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Fellowship
• 财政年份: 2006
• 资助国家: 英国
• 起止时间: 2006 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FC535405%2F1
• 关键词: Design; tissue; engineering; bioreactors; using

Proposed here, is a study of bioreactor design using cultures of rat liver cells which were chosen for ease of handling, safety and their ability to regenerate quickly over short periods of time. This will be done across two length scales. Firstly, Two-Photon Microscopy (TPM) will be used to study the impact of bioreactor design on the cellular interactions and growth on a submicron length scale. Secondly, the hydrodynamics of the bioreactors and its impact on the tissue will be studied on the micron scale using Magnetic Resonance Imaging (MRI). Both these techniques are non-invasive and allow for the bioreactor to be studied in-situ. I believe that using a combination of the two techniques will allow the necessary information on the chemistry, physics and biology characterising these systems, over the hierarchy of length scales relevant to reactor design, to be obtained for the first time.Throughout the course of this project, my current expertise in MRI and chemical engineering will be complemented by the new skills I will learn in tissue engineering and two photon microscopy. The synergy of these skills and techniques will be the basis for detailed hydrodynamic studies, optimisation and scale-up.

这里提出的是一项使用大鼠肝细胞培养物的生物反应器设计研究，选择这些细胞是为了便于处理、安全性和在短时间内快速再生的能力。这将在两个长度尺度上完成。首先，双光子显微镜（TPM）将用于研究生物反应器设计对亚微米长度尺度上的细胞相互作用和生长的影响。其次，将使用磁共振成像（MRI）在微米尺度上研究生物反应器的流体动力学及其对组织的影响。这两种技术都是非侵入性的，并允许原位研究生物反应器。我相信，使用这两种技术的组合将允许在与反应堆设计相关的长度尺度的层次上首次获得表征这些系统的化学、物理和生物学的必要信息。我目前在核磁共振成像和化学工程方面的专业知识将得到我将在组织工程和双光子显微镜方面学习的新技能的补充。这些技能和技术的协同作用将成为详细的流体动力学研究、优化和扩大规模的基础。