A Zeiss Duo Confocal Microscope for Shared Imaging Facility

我们建议获得一个最近开发的，双重配置的共聚焦显微镜（LSM 5Duo，蔡司） 为了支持马里兰州大学医学院9名NIH资助的研究人员的研究， 药这些研究人员都迫切需要高速，多色荧光共聚焦 在光活化、光漂白或长距离Z运动期间成像。没有配备所需的 能力存在于校园中。蔡司Duo结合了成熟的高分辨率光学 LSM 510的切片能力与较新的LSM的高速成像能力 5Live. Duo的两个独立的光束转向系统创造了协同能力， 在快速图像采集期间同时进行感兴趣区域光操纵。各大用户 证实了Duo的能力直接且独特地满足了他们的要求。新 仪器将被纳入该大学长期存在的共焦核心设施，在那里它将 取代1994年购买的LSM 410，该LSM 410既不适合活细胞，也不可靠 实验，现在占据了大部分的时间在共聚焦显微镜的设施。后 评估来自主要供应商的可能的系统配置，设施？监督委员会还 一致认为Duo是唯一能够满足日益增长的高速需求的配置， 活细胞成像，同时提供一致的访问高分辨率点扫描共聚焦。 因此，机构支持是非常强大的院长？的水平，也广泛传播到许多 拥有大量NIH支持的研究的部门。共焦核心中的二重奏 该设施将直接和有力地有利于主要用户的研究;更广泛地说，它将增加一个 重要的新能力，促进NIH赞助的研究，许多其他研究人员对 研究活细胞和系统中的生物过程。

We propose to acquire a recently developed, dually configured confocal microscope (the LSM 5 Duo, Zeiss) to support the research of a group of 9 NIH-funded investigators at the University of Maryland School of Medicine. These investigators each have immediate need for high-speed, multi-color fluorescence confocal imaging during photoactivation, photobleaching, or long-distance Z motion. No equipment with the required capabilities exists on campus. The Zeiss Duo combines the well-established, high-resolution optical sectioning capabilities of the LSM 510 with the high-speed imaging capabilities of the more recent LSM 5Live. The two, independent beam-steering systems of the Duo create the synergistic capacity for simultaneous region-of-interest photomanipulation during rapid image acquisition. The Major Users each confirmed that the capabilities of the Duo directly and uniquely well meet their requirements. The new instrument will be incorporated into the long-standing Confocal Core Facility at the University, where it will replace an LSM410 that was purchased in 1994 and is neither suitable nor reliable for the live-cell experiments that now occupy the large majority of time on the confocal microscopes in the Facility. After evaluating possible system configurations from the major vendors, the Facility?s oversight committee also agreed that the Duo presented the only configuration that would meet the increasing demand for highspeed, live-cell imaging while providing consistent access to a high-resolution point-scanning confocal. Accordingly, institutional support is very strong at the Dean?s level and also spread widely among many Departments with extensive NIH-supported research. The presence of the Duo in the Confocal Core Facility would directly and strongly benefit the research of the Major Users; more broadly, it would add an important new capability for advancing the NIH-sponsored research of the many other investigators on campus studying biological processes in living cells and systems.