Brown University Shared DNA Sequencer

描述(申请人提供)：本申请建议用最先进的机器取代过时的DNA测序技术(制造商于6/30/06停止使用)。这项提议的广泛目标是通过将分子遗传学与目前正在收集的密集的表型和环境数据相结合，丰富布朗大学和合作机构正在进行的精神病学和行为研究。IS提案的具体目标是用最先进的应用生物系统3130xl遗传分析仪取代不再受支持的应用生物系统377 DNA测序仪。获得新的遗传分析仪将允许在确定药物效果的遗传调节剂(例如，McGeary等人，2004年)、确定基因对环境相互作用的遗传贡献(例如，Gibb等人，2006年)和确定精神亚型(例如，McGeary等人，2006年)方面取得不间断的进展。目前将从这一收购中受益的研究项目包括许多治疗酒精和尼古丁依赖的药物试验，目的是通过识别预测阳性反应(良好治疗结果)和阴性反应(副作用)的基因变异来改进药物治疗。目前有19项研究提案正在审查中，这些提案将受益于获得这种设备。这些项目涉及多种方法，包括神经成像评估研究、对多种滥用药物的人体实验室挑战研究、肥胖症、抑郁症和焦虑症的评估，以及基因对人类睡眠影响的调查。

DESCRIPTION (provided by applicant): This application proposes to replace outdated DNA sequencing technology (discontinued by manufacturer on 6/30/06) with a state of the art machine. The broad objectives of this proposal are to enrich ongoing psychiatric and behavioral research at Brown University and collaborating institutions through the integration of molecular genetics with the intensive phenotypic and environmental data currently being collected. The specific aim of the is proposal is to replace the no-longer supported Applied Biosystems 377 DNA Sequencer with a state-of-the-art Applied Biosystems 3130xl Genetic Analyzer. Obtaining a new genetic analyzer would allow uninterrupted progress in identifying genetic moderators of drug's effects (e.g., McGeary et al 2004), defining genetic contributions to gene by environment interactions (e.g., Gibb et al, 2006) and defining psychiatric subtypes (e.g., McGeary et al., 2006). Current research projects that would benefit from this acquisition include numerous trials of medications for alcohol and nicotine dependence with the goal of improving pharmacological treatments through the identification of genetic variation that predicts positive response (good treatment outcome) and negative response (side effects). There are 19 research proposals currently under review that would benefit from access to this equipment. These projects span multiple methodologies and include neuroimaging assessment studies, human lab challenge studies of multiple drugs of abuse, assessments of obesity, depression and anxiety disorders and investigations of genetic influences on human sleep.