PROTEOMICS SHARED RESOURCE

蛋白质组学共享资源为癌症研究提供最先进的蛋白质技术， 侧重于质谱和分析化学的应用。服务包括：蛋白质鉴定 从溶液中或1D或2D凝胶中的样品; 2D凝胶电泳，2D-DIGE，图像分析，斑点拾取， 斑点消化;蛋白质复合物的质谱分析;膜蛋白;细胞器 蛋白质组和亚蛋白质组;不同实验条件下蛋白质表达的定量分析 条件;翻译后修饰;蛋白质的自上而下分析;生物标志物的开发， 发现项目;通过LC-MS进行小分子分析，以及蛋白质的氢氘交换研究 动力学有一个相邻的计算区，用于离线质谱数据分析， 搜索和数据挖掘。自上次审查以来的主要事态发展包括对整个 蛋白质组学设施和冷却设备的安装，以稳定仪器的温度。四 沿着新的质谱仪（TOFTOF、LTQ线性阱、FT-ICR、QSTAR XL）， 台风荧光扫描仪。还招聘了几名新的高素质工作人员。 其中包括：傅里叶变换红外光谱质谱和氢氘交换方面的专家Hui Xiao博士 从阿默斯特的马萨诸塞州大学招募;同样来自马萨诸塞大学的Jihyeon Lim博士，阿默斯特， 质谱专家和定量蛋白质组学专家车发云博士。肽合成 2005年年底，基于来自以下公司的高质量肽的可用性， 商业部门，以便更有效地利用人员来支持其他关键服务， 用户优先。该设备在各种质谱应用中发挥了重要作用 支持高度优先的AECC倡议。这些包括过渡态酶抑制剂的开发， 微管蛋白异构体和微管抑制剂结合位点的研究，蛋白质组学分析作为预测 乳腺癌风险和预测头部鳞状细胞癌的治疗反应， 脖子该机构一直积极开发新方法来满足AECC调查员的要求 这一举措有助于满足需求，并培训AECC调查人员，使他们能够使用开放获取的实验室设备。

The Proteomics Shared Resource provides state-of-the-art protein technologies for cancer research with a focus on application of mass spectrometry and analytical chemistry. Services include: protein identification from samples in solution or in 1D or 2D gels; 2D gel electrophoresis, 2D-DIGE, image analysis, spot picking, and spot digestion; mass spectrometry analysis of protein complexes; membrane proteins; organellar proteomes and subproteomes; quantitative analysis of protein expression in different experimental conditions; posttranslational modifications; top-down analysis of proteins; development of biomarker and discovery projects; small molecule analysis by LC-MS, and hydrogen-deuterium exchange studies for protein dynamics. There is an adjacent computing area for offline mass spectrometry data analysis plus web-based searches and data mining. Major developments since the last review include a major renovation of the entire Proteomics facility and the installation of cooling equipment for temperature stabilization of instruments. Four new mass spectrometers (TOFTOF, LTQ linear trap, FT-ICR, QSTAR XL) were acquired along with a Typhoon fluorescence scanner. Several new highly qualified staff members have also been recruited. These include: Dr. Hui Xiao, an expert in FT-ICR mass spectrometry and hydrogen-deuterium exchange recruited from the University of Massachusetts, Amherst; Dr. Jihyeon Lim, also of Univ. of Mass., Amherst, an expert mass spectrometrist; and Dr. Fa-Yun Che, an expert in quantitative proteomics. Peptide synthesis was discontinued at the end of 2005 based upon the availability of high-quality peptides from companies in the commercial sector, allowing more effective utilization of personnel to support other critical services of high priority to users. The facility has played an important role in a variety of mass spectrometry applications that support high-priority AECC initiatives. These include development of transition-state enzyme inhibitors, the study of tubulin isoforms and microtubule-inhibitor binding sites, proteomic analyses as a predictor of breast cancer risk and for the prediction of response to treatment in squamous cell cancer of the head and neck. The facility has been active in the development of new methodologies to meet AECC investigator needs and in training AECC investigators so that they can use open-access laboratory equipment.