Imaging Mass Spectrometry System

描述(由申请人提供)：本申请需要一个成像质谱学(IMS)系统，用于对人类健康重要的基础和翻译研究。这一仪器系统将被一组经验丰富的研究人员用于以下研究领域：1)癌症生物学，研究转移和侵袭机制；2)传染病，研究对艾滋病毒、结核病、寄生虫和真菌疾病的免疫和组织特异性反应机制；3)肾脏疾病；4)神经传递；5)生殖生物学。将研究转基因动物模型的组织、外植体和活检组织，以及完整的斑马鱼突变体。所要求的仪器是Bruker Daltonics公司的UltrafleXtreme(Tm)MALDI-TOF/TOF系统，其特点使其非常适合拟议的项目。这些项目将利用UltrafleXtreme(Tm)的能力，并通过使用它来生成和发现它们的假设。该仪器将安装在一个已建立的质谱学资源实验室中，在一个高度互动和支持的环境中，由一名高技能的调查人员在优秀的科学家人员的支持下进行监督。该仪器系统还将使最近资助的爱因斯坦NIDA蛋白质组学中心受益，该中心研究艾滋病毒和药物滥用的神经学后果。

DESCRIPTION (provided by applicant): This application requests an imaging mass spectrometry (IMS) system for basic and translational research important to human health. This instrument system will be used by a group of experienced investigators in the following research areas: 1) cancer biology, to investigate mechanisms of metastasis and invasion; 2) infectious diseases, to study the mechanisms of immune and tissue specific response to HIV, Tb, parasitic and fungal diseases; 3) renal disease; 4) neurotransmission; and 5) reproductive biology. Tissues from transgenic animal models, explants, and biopsies will be studied, as well as intact zebrafish mutants. The requested instrument is an UltrafleXtreme(tm) MALDI- TOF/TOF system from Bruker Daltonics that has features making it ideally suited for the projects proposed. The projects will take advantage of the capabilities of the UltrafleXtreme(tm), and be enabled in their hypothesis generation and discovery by its use. This instrument will be located in an established mass spectrometry resource laboratory under the supervision of a highly skilled investigator with the support of excellent staff scientists in a highly interactive and supportive environment. The instrument system will also benefit the recently funded Einstein NIDA Proteomics Center on the neurological consequences of HIV and drug abuse.