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中文摘要
翻译
蛋白质组学核心将使用SILAM(先导I,小鼠模型)、SILAC(先导II,细胞培养模型)和iTRAQ(先导II,单核细胞;先导III,人血小板不良血浆)方法为先导项目l-ll提供定量蛋白质组学分析。蛋白质组学核心也将协助为这些研究准备组织和蛋白质。工作将与生物信息学和生物统计学核心进行数据管理,分析和整合。
英文摘要
The Proteomics Core will provide quantitative proteomics analysis for Pilot Projects l-lll using SILAM (pilots I, mouse model), SILAC (pilot II, cell culture model), and iTRAQ (pilot II, monocytes; pilot III, human platelet poor plasma) approaches. The Proteomics Core will also assist in preparing tissue and protein for these studies. Work will be integrated with the Bioinformatics & Biostatistics Core for data management, analysis and integration.
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Imaging Mass Spectrometry System
LTQ Orbitrap Velos Mass Spectrometer System
QTRAP 5500 Triple Quadrupole Mass Spectrometer System
Pilot Proteomics Center on Drug Abuse HIV/AIDS
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