Proteomics Core

蛋白质组学核心

• 批准号: 7617348
• 负责人: RUTH Hogue ANGELETTI
• 金额: $ 27.28万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2011-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7617348
• 关键词: AIDS/HIV problem; Adhesions; Animals; Antibodies; Archives; Bioinformatics; Biological Markers; Biostatistics Core; Blood Platelets; Brain; Brain region; Buprenorphine; Carbonates; Cell Culture Techniques; Cell surface; Cytosol; Data; Data Analyses; Databases; Drug Addiction; Drug abuse; Experimental Designs; HIV; Hand; Human; Integral Membrane Protein; Investigation; Label; Longitudinal Studies; Mass Spectrum Analysis; Membrane; Methods; Modeling; Neurocognitive; Neuropeptides; Opioid Receptor; Patients; Peptides; Phosphorylated Peptide; Phosphotyrosine; Pilot Projects; Plasma; Preparation; Procedures; Protein Analysis; Proteins; Proteomics; Protocols documentation; Research; Sampling; Serine; Testing; Threonine; Tissues; Vascular Endothelial Cell; Work; cohort; data acquisition; data management; immunoaffinity chromatography; instrument; member; monocyte; mouse model; neurocognitive test; prospective

The Proteomics Core will provide quantitative proteomics analysis for Pilot Projects l-lll using SILAM (pilots I, mouse model), SILAC (pilot II, cell culture model), and iTRAQ (pilot II, monocytes; pilot III, human platelet poor plasma) approaches. The Proteomics Core will also assist in preparing tissue and protein for these studies. Work will be integrated with the Bioinformatics & Biostatistics Core for data management, analysis and integration.

蛋白质组学核心将使用SILAM(Pilot I，小鼠模型)、SILAC(Pilot II，细胞培养模型)和iTRAQ(Pilot II，单核细胞；Pilot III，人血小板贫乏血浆)方法，为试点项目L-11提供定量蛋白质组学分析。蛋白质组学核心还将协助为这些研究准备组织和蛋白质。工作将与生物信息学和生物统计核心相结合，以进行数据管理、分析和整合。