Mass Spectrometer System

描述(申请人提供)：申请资金用于质谱计系统，由ThermoFinnigan LCQ Deca XP Plus超灵敏MS n离子陷阱质谱仪和大气压MALDI离子源组成，带有能进行二维层析的纳升流动高效液相色谱。该仪器系统具有高灵敏度和高质量准确度，并具有串联质谱分析功能。它非常适合于蛋白质组学应用，大分子组装的表征，以及技术转移应用中生物标记物和潜在疗法的模式识别。13个研究小组参与了这项提案，其中包括了解肥胖、减数分裂、信号转导、艾滋病毒/艾滋病、传染病和癌症的机制的项目。这些仪器将由经验丰富的质谱学家和蛋白质化学家在现有的共享蛋白质核心设施内操作和监督，该设施具有生产力和协作相互作用的记录。

DESCRIPTION (provided by applicant): Funds are requested for a mass spectrometer system, composed of a ThermoFinnigan LCQ Deca XP Plus ultra sensitive MS n ion trap mass spectrometer and atmospheric pressure MALDI ion source, with a nanoliter flow HPLC capable of two-dimensional chromatography. This instrument system provides high sensitivity and mass accuracy with tandem mass spectrometry capabilities. It is ideally suited for proteomics applications, characterization of macromolecular assembles, and identification of patterns of biomarkers and potential therapeutics in technology transfer applications. Thirteen groups of investigators participate in this proposal, which includes projects in understanding the mechanisms of obesity, meiosis, signal transduction, HIV/AIDS, infectious diseases and cancer. The instruments will be operated and supervised by experienced mass spectrometrists and protein chemists within an existing shared protein core facility with a record of productivity and collaborative interactions.