Exploitation of new lanthanide technology

新型稀土元素技术开发

• 批准号: EP/G004773/1
• 负责人: David Parker
• 金额: $ 12.55万
• 依托单位: Durham University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2008
• 资助国家: 英国
• 起止时间: 2008 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FG004773%2F1
• 关键词: Exploitation; new; lanthanide; technology

This project seeks to develop chemical assays and novel analytical methodology for the determination of selected bioactive species, including tumour markers. The project is based on new chemical technology derived from lanthanide chemistry and is of direct relevance to clinical/biochemical analyses and the development of diagnostic molecular imaging, e.g. for screening of clicial samples for prostate cancer or in urine analysis to monitor metabolite compostion. The key scientific breakthroughs have been made during the course of recent EPSRC project grant support and the project will strive to capitalise on these through licensing and IP transfer agreements with targeted enterprises.Luminescent molecules can be detected with very high sensitivity, down to levels of detecting single molecules, and continue to replace radioactive labels in many applications in the life and clinical sciences. These fluorescent entities should be designed to be chemically stable and must resist processes which quench their fluorescence. They must also be able to localise at the desired target and send information to the observer that signals where they are. The industry requires new systems that emit a longer-lived fluorescent signal which encodes information about the nature of the local environment. These responsive probes, may for example, emit light at two or three different wavelengths, and the relative intensity of the emitted light at these wavelengths is indicative of the local concentration of selected bioactive species. Complexes have been identified that not only seek out particular parts of the cell, but can also give encoded information in their fluorescence signal that tells the observer about the local chemical composition of that environment, and how it may change with time and by external perturbation.

该项目旨在开发化学分析和新的分析方法，用于确定选定的生物活性物质，包括肿瘤标志物。该项目基于镧系元素化学衍生的新化学技术，与临床/生物化学分析和诊断分子成像的开发直接相关，例如用于筛查前列腺癌的临床样本或用于尿液分析以监测代谢物成分。在最近的EPSRC项目赠款支持过程中，已经取得了关键的科学突破，该项目将努力通过与目标企业的许可和知识产权转让协议来利用这些突破。发光分子可以以非常高的灵敏度检测，低至检测单个分子的水平，并继续在生命和临床科学的许多应用中取代放射性标记。这些荧光实体应被设计成化学稳定的，并且必须抵抗淬灭其荧光的过程。它们还必须能够定位于所需的目标，并向观察者发送信息，表明它们的位置。该行业需要发射更长寿命的荧光信号的新系统，该信号编码有关当地环境性质的信息。这些响应探针可以例如发射两个或三个不同波长的光，并且在这些波长下发射的光的相对强度指示所选生物活性物质的局部浓度。已经发现的复合物不仅可以寻找细胞的特定部分，而且还可以在其荧光信号中提供编码信息，告诉观察者该环境的局部化学成分，以及它如何随时间和外部扰动而变化。

项目成果

Cell penetrating metal complexes as optical probes: a mechanistic approach to targeted responsive systems based on lanthanide luminescence

作为光学探针的细胞穿透金属配合物：基于镧系元素发光的靶向响应系统的机械方法

• 发表时间: 2009
• 期刊: Accounts of Chemical Research
• 影响因子: 18.3
• 作者: D Parker

A europium luminescence assay of lactate and citrate in biological fluids.

• DOI: 10.1039/b901251f
• 发表时间: 2009-04-21
• 期刊: Organic & biomolecular chemistry
• 影响因子: 3.2
• 作者: Pal R;Parker D;Costello LC
• 通讯作者: Costello LC

Analysis of citrate in low-volume seminal fluid samples using a time-gated measurement of europium luminescence.

使用铕发光的时间选通测量分析少量精液样品中的柠檬酸盐。

• DOI: 10.1016/j.jpba.2011.05.023
• 发表时间: 2011
• 期刊: Journal of pharmaceutical and biomedical analysis
• 影响因子: 3.4
• 作者: Pal R

Critical evaluation of five emissive europium(III) complexes as optical probes: correlation of cytotoxicity, anion and protein affinity with complex structure, stability and intracellular localisation profile

• DOI: 10.1039/b803895c
• 发表时间: 2008-01-01
• 期刊: ORGANIC & BIOMOLECULAR CHEMISTRY
• 影响因子: 3.2
• 作者: Murray, Benjamin S.;New, Elizabeth J.;Parker, David
• 通讯作者: Parker, David