BIOENGINEERING PRIMATE FOLLICLES: FROM IMMATURE EGGS TO LIVE BIRTHS

灵长类卵泡生物工程：从未成熟卵子到活产

• 批准号: 7958468
• 负责人: RICHARD L STOUFFER
• 金额: $ 10.04万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-04 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7958468
• 关键词: Abdomen; Alginates; Angiogenic Factor; Architecture; Autologous Transplantation; Biocompatible Materials; Biological Preservation; Biomedical Engineering; Biopsy; Cancer Survivor; Clinical; Computer Retrieval of Information on Scientific Projects Database; Cortex of ovary; Development; Embryo; Embryo Transfer; Exposure to; Female; Fertility; Fertilization; Fertilization in Vitro; Forearm; Funding; Germ Cells; Goals; Graafian Follicles; Grant; Growth; Growth Factor; Health; Hormones; In Vitro; Institution; Live Birth; Macaca; Monitor; Monkeys; Mothers; Oocytes; Ovarian; Ovarian Tissue; Ovary; Pregnancy; Primates; Research; Research Personnel; Resources; Role; Site; Source; Transplantation; United States National Institutes of Health; Universities; Woman; egg; in vivo; nonhuman primate; offspring; oocyte maturation; prevent; reproductive; scaffold

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project is part of a Roadmap Consortium (U54 RR02437, T. Woodruff, PI) entitled "The Oncofertility Consortium: Fertility Preservation for Women". This project's goal is to validate options in nonhuman primates for restoring fertility in female cancer survivors by preventing ovarian exposure to the gametotoxic effects of therapy (by removing and preserving biopsies) and returning ovarian tissue, gametes or embryos for fertility. Studies will be performed in monkeys to: (Aim 1) Bioengineer a scaffold that supports the three-dimensional architecture of the follicle and permits coordinated development of the follicle wall and oocyte in vitro; (Aim 2) Evaluate the role of hormones and growth factors in promoting follicle growth and oocyte quality during culture; (Aim 3) Optimize conditions for autotransplantation of ovarian cortex for coordinated follicle growth and oocyte maturation in vivo; and (Aim 4) Assess fertilization and embryonic potential of oocytes derived from in vitro matured follicles and transplanted ovarian cortical follicles in vivo. Immature follicles will be isolated from macaque ovaries and cultured in an alginate matrix. Ovarian cortex will be autotransplanted to accessible sites (abdomen and forearm), and the ability of pro-angiogenic factors to restore ovarian function will be monitored. Mature oocytes produced by follicles in vitro and cortical transplants in vivo will be evaluated for reproductive potential by in vitro fertilization, embryo transfer into surrogate macaque mothers, pregnancy and health of offspring. This project is facilitated by interactions with the Biomaterials Core at Northwestern University. Expected advances will be transferred to clinical efforts at the Fertility Centers.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该项目是路线图联盟（U 54 RR 02437，T。Woodruff，PI），题为“The Oncofertility Consortium：Fertility Preservation for Women”。该项目的目标是验证非人类灵长类动物中恢复女性癌症幸存者生育能力的选择，方法是通过防止卵巢暴露于治疗的配子毒性作用（通过去除和保存活检）并返回卵巢组织，配子或胚胎用于生育。 将在猴中进行研究，以：（目标1）生物工程化支持卵泡三维结构并允许卵泡壁和卵母细胞在体外协调发育的支架;（目标2）评价激素和生长因子在培养期间促进卵泡生长和卵母细胞质量的作用;（3）优化自体卵巢皮质移植条件，促进卵泡生长和卵母细胞成熟;目的4评价体外成熟卵泡卵母细胞和皮质卵泡卵母细胞移植后的受精和胚胎发育潜能。 将从猕猴卵巢中分离未成熟卵泡，并在藻酸盐基质中培养。 将卵巢皮质自体移植到可触及部位（腹部和前臂），并监测促血管生成因子恢复卵巢功能的能力。 将通过体外受精、胚胎移植至替代猕猴母体、妊娠和后代健康来评价体外卵泡和体内皮质移植产生的成熟卵母细胞的生殖潜力。 该项目是通过与西北大学生物材料核心的互动来促进的。 预期的进展将转移到生育中心的临床工作中。