GENE EXPRESSION IN THE PRIMATE OVULATORY FOLLICLE

灵长类动物排卵卵泡中的基因表达

• 批准号: 8357747
• 负责人: RICHARD L STOUFFER
• 金额: $ 0.31万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357747
• 关键词: Adult; Cells; Contraceptive Agents; Dinoprostone; Enzymes; Event; Fertility; Funding; Gene Expression; Gonadotropins; Graafian Follicles; Grant; Hormones; In Vitro; Laboratories; Luteinizing Hormone; Macaca mulatta; Meiosis; Menstrual cycle; Messenger RNA; Monkeys; National Center for Research Resources; Oocytes; Ovary; Ovulation; Pituitary Gland; Primates; Principal Investigator; Process; Prostaglandin E Receptor; Prostaglandins; Receptor Signaling; Research; Research Infrastructure; Resources; Rodent; Rupture; Source; United States National Institutes of Health; cost; design; egg; in vivo; oocyte maturation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In mammalian species, the midcycle surge of gonadotropin hormone (notably luteinizing hormone, LH) secreted by the pituitary gland, initiates a cascade of events in the ovary leading to ovulation of the mature follicle (i.e., rupture of the follicle wall and release of its enclosed oocyte),.. As part of the process, the gonadotropin surge acts on the preovulatory follicle to elicit cumulus-oocyte maturation, including reinitiation of meiosis in the oocyte and expansion of the surrounding cumulus cells, thereby producing a fertilizable egg that can be released from the follicle at ovulation. Rodent studies suggest that prostaglandin (PG) synthesis and local PGE2-receptor (via PGER2 not R1) action is critical for periovulatory events, including cumulus-oocyte expansion (C-OE), and fertility. Studies performed in the Stouffer laboratory demonstrated significant increases in mRNA levels for the enzymes involved in PGE synthesis, as well as PGER2, in the periovulatory follicle of rhesus macaques. Therefore, further studies were designed to evaluate whether a PGE2 antagonist, compared to vehicle control: (1) altered timely ovulation during the natural menstrual cycle, and (2) blocked C-OE in the periovulatory follicle, in rhesus monkeys. In-vivo and in vitro studies suggest that PGE2-receptor signaling promotes C-OE in primates, and provided the rationale for a new project to determine if a PGE2 antagonist was an effective, reversible contraceptive in adult, cycling monkeys.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 在哺乳动物中，脑下垂体分泌的促性腺激素(特别是促黄体激素)在周期中期激增，在卵巢中启动一系列事件，导致成熟卵泡排卵(即卵泡壁破裂并释放其封闭的卵母细胞)。作为这一过程的一部分，促性腺激素激增作用于排卵前的卵泡，诱导卵丘-卵母细胞成熟，包括重新启动卵母细胞的减数分裂和周围卵丘细胞的扩张，从而产生可以在排卵时从卵泡中释放的受精卵。啮齿类动物的研究表明，前列腺素(PG)的合成和局部PGE2受体(通过PGER2而不是R1)的作用对包括卵丘-卵母细胞扩张(C-OE)在内的排卵事件和生育至关重要。在Stouffer实验室进行的研究表明，恒河猴排卵周围卵泡中参与PGE合成的酶以及PGER2的mRNA水平显著增加。因此，进一步的研究旨在评估PGE2拮抗剂与赋形剂对照组相比：(1)改变自然月经周期中的适时排卵，(2)阻断排卵周围卵泡中的C-OE。体内和体外研究表明，PGE2受体信号转导促进灵长类动物的C-OE，并为一项新项目提供了理论基础，该项目旨在确定PGE2拮抗剂是否对成年骑行猴子是有效的、可逆的避孕药。