STRUCTURAL MOLECULAR BIOLOGY SMALL ANGLE X-RAY SCATTERING STATION BEAM LINE 4-2

结构分子生物学小角度X射线散射站束线4-2

• 批准号: 7721863
• 负责人: HIROTSUGU TSURUTA
• 金额: $ 4.02万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721863
• 关键词: Arts; Biological; Cells; Computer Retrieval of Information on Scientific Projects Database; Data; Data Collection; Development; Fiber; Funding; Grant; Improve Access; Incidence; Institution; Membrane Lipids; Molecular Biology; Optics; Process; Proteins; Research; Research Personnel; Resources; Sampling; Schedule; Series; Solutions; Source; System; Testing; United States National Institutes of Health; Vacuum; Virion; X-Ray Crystallography; detector; improved; instrument; instrumentation; novel; research study; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The SSRL Structural Molecular Biology small-angle x-ray scattering station BL4-2 is dedicated to structural biology studies, providing state-of-the-art experimental facilities for structural biology studies on proteins, protein assemblies, virus particles, biological fibers and lipid membranes. The in-hutch instrumentation, featuring a pin-hole geometry small angle scattering camera with an automated distance change mechanism, can be configured to accommodate several data collection modes, including solution scattering, single crystal diffraction, and gracing-incidence scattering. A portable high angle setup is used for fiber diffraction studies to reach 2.4 A in Bragg spacing. New developments this year include a series of experiments to evaluate several types of thin windows for sample cells and vacuum isolation; testing of a novel container-less solution scattering experimental geometry; and continued development of an automated high-throughput solution scattering data collection system. The station is undergoing a 500-mA SPEAR3 optics upgrade and the is perforemd through a relocation to a new beam port. The new BL4-2 expermental hutch has been built and the installation of optic components is in progress. The SMB SAXS team is in the process of upgrading the pin-hole camera to have shorter and longer distances for improved access to both high and very low scattering angles. A new high-efficiency detector, MarMosaic225-HE (funded through DOE BER) is scheduled to arrive in January 2008. Together with the new higher beam flux level, improved beam stability and the new detector, the upgraded instrument will enable much improved data at lower protein concentrations at higher angles as well as at smaller angles, beginning in the spring of 2008.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 SSRL结构分子生物学小角X射线散射站BL4-2致力于结构生物学研究，为蛋白质、蛋白质组件、病毒颗粒、生物纤维和脂膜的结构生物学研究提供最先进的实验设施。该仪器采用针孔几何小角散射相机，具有自动改变距离的机制，可配置为支持多种数据采集模式，包括溶液散射、单晶衍射和光入射散射。便携式高角装置用于光纤衍射研究，在布拉格间距达到2.4A。今年的新进展包括一系列实验，以评估用于样品池和真空隔离的几种类型的薄窗口；测试一种新的无容器溶液散射实验几何；以及继续开发自动化的高通量溶液散射数据收集系统。空间站正在进行500毫安的SPEAR3光学系统升级，并将通过搬迁到新的波束端口进行升级。新的BL4-2试验室已经建成，光学部件的安装正在进行中。SMB SAXS团队正在升级针孔相机，使其具有更短和更长的距离，以便更好地访问高散射角和极低散射角。一种新的高效探测器MarMosaic225-HE(由能源部BER资助)计划于2008年1月到达。与新的更高的束流水平、改进的束流稳定性和新的探测器一起，升级后的仪器将从2008年春季开始，在蛋白质浓度较低的情况下，在更高角度和更小角度下实现更好的数据。