STRUCTURAL MOLECULAR BIOLOGY SMALL ANGLE X-RAY SCATTERING STATION BEAM LINE 4-2

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 SSRL Structural Molecular Biology小角X射线散射站BL 4 -2致力于结构生物学研究，为蛋白质、蛋白质组装体、病毒颗粒、生物纤维和脂质膜的结构生物学研究提供最先进的实验设施。该仪器配备了一个针孔几何小角度散射相机，具有自动距离变化机制，可配置为适应多种数据收集模式，包括溶液散射、单晶衍射和倾斜入射散射。一个便携式高角度设置用于光纤衍射研究，达到2.4 A的布拉格间距。今年的新进展包括一系列实验，以评估用于样品池和真空隔离的几种类型的薄窗口;测试一种新型的无容器溶液散射实验几何形状;以及继续开发自动化高通量溶液散射数据收集系统。该站正在进行500 mA SPEAR 3光学升级，并通过重新定位到新的光束端口来执行。 新的BL 4 -2实验箱已经建成，光学元件的安装正在进行中。SMB SAXS团队正在升级针孔相机，使其具有更短和更长的距离，以改善对高散射角和极低散射角的访问。一个新的高效探测器MarMosaic 225-HE（由能源部BER资助）计划于2008年1月抵达。加上新的更高的光束通量水平，改进的光束稳定性和新的检测器，升级后的仪器将能够在更高的角度以及在更小的角度，在更低的蛋白质浓度大大改善数据，从2008年春天开始。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The SSRL Structural Molecular Biology small-angle x-ray scattering station BL4-2 is dedicated to structural biology studies, providing state-of-the-art experimental facilities for structural biology studies on proteins, protein assemblies, virus particles, biological fibers and lipid membranes. The in-hutch instrumentation, featuring a pin-hole geometry small angle scattering camera with an automated distance change mechanism, can be configured to accommodate several data collection modes, including solution scattering, single crystal diffraction, and gracing-incidence scattering. A portable high angle setup is used for fiber diffraction studies to reach 2.4 A in Bragg spacing. New developments this year include a series of experiments to evaluate several types of thin windows for sample cells and vacuum isolation; testing of a novel container-less solution scattering experimental geometry; and continued development of an automated high-throughput solution scattering data collection system. The station is undergoing a 500-mA SPEAR3 optics upgrade and the is perforemd through a relocation to a new beam port. The new BL4-2 expermental hutch has been built and the installation of optic components is in progress. The SMB SAXS team is in the process of upgrading the pin-hole camera to have shorter and longer distances for improved access to both high and very low scattering angles. A new high-efficiency detector, MarMosaic225-HE (funded through DOE BER) is scheduled to arrive in January 2008. Together with the new higher beam flux level, improved beam stability and the new detector, the upgraded instrument will enable much improved data at lower protein concentrations at higher angles as well as at smaller angles, beginning in the spring of 2008.