REFINING THE STRUCTURE OF THE SMALL GAMMA TUBULIN COMPLEX

细化小伽玛微管蛋白复合物的结构

• 批准号: 7723738
• 负责人: DAVID A. AGARD
• 金额: $ 1.95万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7723738
• 关键词: Binding; Complex; Computer Retrieval of Information on Scientific Projects Database; Fluorescence Resonance Energy Transfer; Funding; Goals; Grant; Institution; Link; Microtubules; Research; Research Personnel; Resources; Source; Structure; United States National Institutes of Health; beta Tubulin; gamma Tubulin; in vivo; research study; spindle pole body; yeast two hybrid system

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The small gamma tubulin complex is part of the microtubule-nucleating unit. The complex consists of two Tub4p and one each of Spc97p and Spc98p. Tub4p, gamma tubulin, binds the alpha-beta tubulin heterodimer, the building blocks of microtubules. Two-hybrid experiments have shown that Spc110 interacts with Spc98 and Spc97 and weakly with Tub4. Spc110 is able to directly bind Spc98 in IP experiments from baclovirus extracts. Spc110 provides the link between the gamma tubulin complex and the inner plaque of the spindle pole body (SPB) through its interaction with Spc97 and Spc98. Our goal is to use FRET to refine the structure of the Tub4p complex in vivo.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 小伽玛微管蛋白复合物是微管成核单元的一部分。该复合体由两个 Tub4p 以及 Spc97p 和 Spc98p 各一个组成。 Tub4p（γ 微管蛋白）与 α-β 微管蛋白异二聚体（微管的组成部分）结合。两种杂交实验表明，Spc110 与 Spc98 和 Spc97 相互作用，与 Tub4 相互作用较弱。在 IP 实验中，Spc110 能够直接结合来自杆状病毒提取物的 Spc98。 Spc110 通过与 Spc97 和 Spc98 的相互作用，在 γ 微管蛋白复合物和纺锤体极体 (SPB) 的内部斑块之间建立联系。 我们的目标是利用 FRET 来完善体内 Tub4p 复合物的结构。