IN VIVO DIFFUSION AND SPECTROSCOPIC BRAIN IMAGING IN ALCOHOLISM

酗酒的体内扩散和脑光谱成像

• 批准号: 7722858
• 负责人: Adolf Pfefferbaum
• 金额: $ 1.12万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-01 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722858
• 关键词: Abstinence; Age; Aging; Alcoholism; Algorithms; Area; Attenuated; Brain; Brain imaging; Chronic; Computer Retrieval of Information on Scientific Projects Database; Condition; Corpus Callosum; Data; Diffusion; Equilibrium; Exhibits; Funding; Gait; Grant; Gray unit of radiation dose; Institution; Liquid substance; Magnetic Resonance Imaging; Measures; Methods; Microscopic; Pattern; Performance; Recovery; Relapse; Research; Research Personnel; Resources; Sampling; Scanning; Short-Term Memory; Slice; Source; Tissues; United States National Institutes of Health; Ventricular; Visuospatial; Woman; age related; drinking; follow-up; gray matter; in vivo; men; problem drinker; sex; sobriety; white matter

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Aims: Use DTI and conventional MRI to examine the brain of chronic alcoholics through abstinence and/or relapse. Methods: We studied 57 alcoholics (40 m, 17 w) and 74 controls (32 m, 42 w) 3 months post-treatment. 19 alcoholics and 37 controls returned 2 years later. Of the alcoholics, 12 had maintained sobriety for at least one year prior to follow-up and 7 had resumed drinking. A single shot spin-echo echo-planar coronal DTI sequence, combined with fast spin-echo (FSE), SPoiled Gradient Recalled Echo (SPGR), and Fluid Attenuated Inversion Recovery (FLAIR) structural sequences at 1.5T assessed the interaction of aging and alcoholism on microscopic white matter and macroscopic changes. We segmented SPGR data to assess global volumes of cortical and subcortical gray matter, white matter and CSF and measured area of corpus callosum on a midsagittal slice. From the DTI we computed FA and from geometrically defined regions of corpus callosum as well as manually identified fully-volumed, focal 5 mm3 samples. Results: Control men and women both exhibited age-related increases in CSF and decreases in tissue volumes, especially for gray matter. Volumes for controls and alcoholics were statistically adjusted for the variance associated with ICV and age measured in the controls. Alcoholic men and women had gray and white matter volume tissue deficits and CSF enlargement compared with sex-matched controls. Alcoholic men and women showed similar patterns and extents of callosal shrinkage, which was greatest in the genu and body and less prominent in the splenium. This shrinkage was accompanied by abnormalities in FA and more prominent in the genu than the splenium. Older alcoholics had smaller genu and splenium and higher diffusivity in these regions than younger alcoholics. Significant correlations between regional MRI and DTI measures and performance on working memory, visuospatial ability, and gait and balance provided evidence for the functional ramifications of the callosal abnormalities. On follow-up, there was a significant increase in for relapsers compared to abstainers in the genu, but not the splenium. Using a nonrigid registration algorithm to compare entry and follow-up scans, we observed modest ventricular expansion in controls, more prominent ventricular and fissure expansion in the relapsers. Abstainers showed either conrol-like ventricular expansion (women) or modest evidence for contraction (men). Conclusions: At baseline after three months abstinence, alcoholism interacted with age to exerted a compounded untoward effect on callosal macrostructure and microstructure with frontal distribution and functional ramifications. Sobriety at follow-up was associated with modest improvements in the condition of white matter microstructure and macrostructure.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 目的：利用弥散张量成像（DTI）和常规磁共振成像（MRI）对慢性酒精依赖者的大脑进行研究。 方法：我们研究了57名酗酒者（40个月，17周）和74名对照者（32个月，42周）治疗后3个月。19名酗酒者和37名对照者在两年后返回。 在酗酒者中，12人在随访前至少保持清醒一年，7人恢复饮酒。 单次激发自旋回波回波平面冠状面DTI序列，结合快速自旋回波（FSE），SPGR和液体衰减反转恢复（FLAIR）结构序列在1.5 T下评估了衰老和酒精中毒对微观白色物质和宏观变化的相互作用。 我们对SPGR数据进行分段，以评估皮质和皮质下灰质、白色物质和CSF的整体体积，并在正中矢状切片上测量胼胝体面积。 从DTI，我们计算FA和从几何定义的胼胝体区域，以及手动识别的全容积，局灶性5 mm 3的样本。 结果：对照组男性和女性均表现出年龄相关的CSF增加和组织体积减少，尤其是灰质。 对照组和酗酒者的ICV与对照组中测量的年龄相关的方差进行了统计学调整。 与性别匹配的对照组相比，酗酒的男性和女性有灰色和白色物质体积组织缺损和CSF增大。酗酒的男性和女性表现出相似的胼胝体萎缩模式和程度，这是最大的在躯干和身体和不太突出的压部。 这种萎缩伴随着FA的异常，并且在小脑中比压部更突出。年长的酗酒者在这些区域的酒精浓度和压浓度都比年轻的酗酒者小，扩散率也高。 区域MRI和DTI测量与工作记忆、视觉空间能力、步态和平衡性能之间的显著相关性为胼胝体异常的功能性后果提供了证据。 在随访中，与戒酒者相比，复吸者在青少年中有显著增加，但在压部没有。 使用非刚性配准算法比较入口和后续扫描，我们观察到适度的心室扩张在控制，更突出的复发者的心室和裂隙扩张。 戒断者表现出类似于控制器的心室扩张（女性）或适度的收缩（男性）。 结论：戒酒三个月后，在基线时，酒精中毒与年龄相互作用，对胼胝体的宏观结构和微观结构产生复合不良影响，并对额叶分布和功能产生影响。 随访时清醒与白色物质微观结构和宏观结构状况的适度改善相关。