Genetic Biochemical Studies of Plant Steroid Signaling

植物类固醇信号传导的遗传生化研究

• 批准号: 10254337
• 负责人: ZHIYONG WANG
• 金额: $ 56.14万
• 依托单位: CARNEGIE INSTITUTION OF WASHINGTON, D.C.
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10254337
• 关键词: Animals; Arabidopsis; Automobile Driving; Binding; Biochemical; Biological; Biological Models; Carbon; Cell Cycle Progression; Cell division; Cell physiology; Cells; Cellular biology; Cytokinesis; Development; Developmental Process; Ecosystem; Environmental Risk Factor; Enzymes; Equilibrium; Excision; Family; Food Supply; Gene Expression Regulation; Genetic; Genetic Transcription; Genomics; Gluconeogenesis; Goals; Grant; Growth; Health; Homeostasis; Hormonal; Hormones; Human; Investigation; Label; Link; Mass Spectrum Analysis; Mediating; Membrane; Metabolic; Metabolism; Methodology; Molecular; Nutrient; Organ; Organism; Personal Satisfaction; Phosphoric Monoester Hydrolases; Phosphorylation; Phosphotransferases; Physiological; Physiological Processes; Plants; Play; Post-Translational Protein Processing; Process; Productivity; Protein Phosphatase 2A Regulatory Subunit PR53; Proteomics; Regulation; Research; Research Support; Role; Signal Pathway; Signal Transduction; Signaling Protein; Steroids; System; Transcriptional Activation; Transmembrane Transport; Vesicle; base; cell growth regulation; food security; glycoproteomics; glycosylation; improved; information processing; member; phosphoproteomics; plant growth/development; programs; protein complex; protein protein interaction; public health relevance; receptor; resilience; steroid hormone; sugar; trafficking; transcription factor

Modified Project Summary/Abstract Section The long-term goal of this project is to understand the molecular networks through which hormones, environmental factors, and nutrients together control plant growth and development. Plants have evolved robust cellular signaling systems to regulate growth and metabolism according to internal status and environmental conditions. Dissecting these plant regulatory systems is important not only for food security and environmental sustainability, but also for understanding cellular regulation in general, as many mechanisms are highly conserved in animals and plants. This research program focuses on the cellular signaling and regulatory network impinged upon by the steroid hormone brassinosteroid (BR), a major growth-promoting hormone that impact a wide range of developmental and physiological processes. Using a combination of genetic, genomic, biochemical, and proteomic approaches in the Arabidopsis model system, we have elucidated molecular mechanisms by which BR binding to receptor kinase BRI1 leads to activation of transcription factor BZR1 through the evolutionarily conserved BSU1 family of phosphatases and GSK3-like kinases. Furthermore, we have revealed at molecular level how the BR signaling pathway is integrated with many other signaling pathways into regulatory networks that control gene transcription program driving cell elongation and various specific developmental programs. Our ongoing research has uncovered many posttranslational mechanisms by which BR signaling directly modulates cellular reorganization and metabolic programs through direct protein-protein interactions and posttranslational modifications. We proposed to focus on these mechanisms that link signaling pathways with major cellular activities such as membrane trafficking, cell division, and carbon metabolism. In addition, we will investigate how BR signaling cross talks with other signaling pathways to balance metabolism with growth demand and to program cellular differentiation in plant development. The research outlined in this proposal will continue to use combinations of genetic and proteomic approaches and will significantly advance our understanding of the molecular mechanisms of cellular signal integration and information processing.

修改项目摘要/摘要部分 该项目的长期目标是了解激素，环境因素和营养物质共同控制植物生长和发育的分子网络。植物已经进化出强大的细胞信号系统，根据内部状态和环境条件来调节生长和代谢。剖析这些植物调节系统不仅对粮食安全和环境可持续性很重要，而且对理解一般的细胞调节也很重要，因为许多机制在动物和植物中是高度保守的。该研究项目的重点是类固醇激素油菜素类固醇（BR），一种主要的生长促进激素，影响广泛的发育和生理过程的细胞信号传导和调控网络。使用遗传学，基因组学，生物化学和蛋白质组学方法的组合在拟南芥模型系统中，我们已经阐明了分子机制，BR结合受体激酶BRI 1导致激活转录因子BZR 1通过进化保守的BSU 1家族的磷酸酶和GSK 3样激酶。此外，我们已经在分子水平上揭示了BR信号通路是如何与许多其他信号通路整合到调控网络中的，这些调控网络控制基因转录程序，驱动细胞伸长和各种特定的发育程序。我们正在进行的研究已经发现了许多翻译后机制，BR信号通过直接的蛋白质-蛋白质相互作用和翻译后修饰直接调节细胞重组和代谢程序。我们建议将重点放在这些机制，连接信号通路与主要的细胞活动，如膜运输，细胞分裂和碳代谢。此外，我们将研究BR信号如何与其他信号通路交叉，以平衡新陈代谢与生长需求，并在植物发育中编程细胞分化。本提案中概述的研究将继续使用遗传学和蛋白质组学方法的组合，并将显着推进我们对细胞信号整合和信息处理的分子机制的理解。