STRUCTURAL DETERMINATION OF PROTEINS IN ARABIDOPSIS

拟南芥中蛋白质的结构测定

• 批准号: 7721622
• 负责人: JOHN LUTE MARKLEY
• 金额: $ 8.91万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721622
• 关键词: Arabidopsis; Computer Retrieval of Information on Scientific Projects Database; Development; Funding; Grant; Institution; Methods; Production; Protein Dynamics; Proteins; Proteome; Research; Research Personnel; Resources; Solutions; Source; Structure; Technology; Testing; Thale Cress Proteins; United States National Institutes of Health; X-Ray Crystallography; novel; protein folding; structural genomics; three dimensional structure

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The major aims of the Center for Eukaryotic Structural Genomics are the development and critical analysis of methods for high-throughput, proteome-scale, eukaryotic protein production, characterization, and structure determination. This technology is being tested and refined through the determination of crystal and solution state three-dimensional structures of proteins from Arabidopsis thaliana. Particular emphasis is placed on proteins whose sequences suggest that they may contain a novel fold, proteins associated with novel functions, or proteins likely to have a known fold but with a function not previously associated with that fold. It is envisioned that most of the structures will be determined by X-ray crystallography, with NMR used for smaller, more dynamic proteins and for smaller protein targets that fail to crystallize.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 真核结构基因组学中心的主要目标是开发和批判性分析高通量，蛋白质组规模，真核蛋白质生产，表征和结构测定的方法。这项技术正在测试和完善，通过确定晶体和溶液状态的三维结构的蛋白质从拟南芥。特别强调的是那些序列表明它们可能含有新折叠的蛋白质、与新功能相关的蛋白质，或者可能具有已知折叠但具有以前与该折叠无关的功能的蛋白质。据设想，大多数结构将通过X射线晶体学确定，NMR用于更小、更动态的蛋白质和未能结晶的更小蛋白质靶。