HYBRID SENSORS & INTEGRATED PLATFORMS

混合传感器

• 批准号: 7721079
• 负责人: PETER JS SMITH
• 金额: $ 3.38万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-12-01 至 2008-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721079
• 关键词: Argon; Calcium; Cells; Computer Retrieval of Information on Scientific Projects Database; Coupling; Data Collection; Detection; Diffusion; Ensure; Epithelial; Facility Construction Funding Category; Fiber; Fiber Optics; Fluorescein; Fluoresceins; Frequencies; Funding; Grant; Hybrids; Institution; Ion-Selective Electrodes; Ionophores; Ions; Lasers; Light; Measures; Microelectrodes; Microscope; Molecular; Optics; Protons; Reporter; Research; Research Personnel; Resolution; Resources; Role; Source; Techniques; Technology; United States National Institutes of Health; base; detector; light emission; new technology; optical fiber; patch clamp; prototype; sensor; voltage

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. As part of the BRC's role as an integrated technology resource, we strive to bring together different experimental approaches, providing parallel data collection from single cells. This effort encompasses hybrid sensors, single microelectrodes combining electrochemical and optic approaches, as well as the construction of integrated platforms. Hybrid Sensors Ion-selective electrodes used in the self-referencing configuration (SERIS probes) are highly sensitive, noninvasive detectors of slow diffusion gradients of ions such as protons or calcium from epithelial sheets or even from single cells. The molecular mechanisms of secretion, however, are usually controlled from within the cell, a domain into which a conventional ion-selective electrode cannot penetrate. To gain simultaneous information about the amount of ion secretion and the mechanisms controlling secretion, a dual probe is under construction. The dual probe consists of an ion-selective electrode with a fiber-optic light carrier inserted through the tip, for delivery of excitation light to an arbitrary reporter molecule. A prototype probe has been constructed, following resolution of several technical issues. To avoid destroying the selectivity of the ionophore, the optical fiber had to be silanized. After silanization, selectivity of the ionophore was unchanged, as measured with both calcium- and proton-selective ionophores. Coupling of excitation light into the optical fiber required vibrating the fiber loading end at high (20 kHz) frequencies, to ensure even delivery of optical modes and reduce laser speckle. The microscope was equipped with fluorescein-based filters to allow delivery of blue laser light (argon ion laser) and detection of green emission light. Integrated Platforms New technologies are more generally accepted when they are combined with existing techniques operating in parallel and provide mutual corroboration. The BRC already maintains combined electrochemical and electrophysiological platforms, the later encompassing single microelectrode, voltage, current and patch clamp.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 作为 BRC 作为综合技术资源的一部分，我们努力将不同的实验方法结合在一起，提供来自单个细胞的并行数据收集。 这项工作包括混合传感器、结合电化学和光学方法的单微电极以及集成平台的构建。 混合传感器 自参考配置中使用的离子选择性电极（SERIS 探针）是高度灵敏的非侵入性检测器，可检测来自上皮片甚至单个细胞的离子（例如质子或钙）的缓慢扩散梯度。 然而，分泌的分子机制通常是从细胞内部控制的，这是传统离子选择性电极无法穿透的区域。 为了同时获得有关离子分泌量和控制分泌机制的信息，正在构建双探针。 双探针由离子选择电极组成，其尖端插入光纤光载体，用于将激发光传递到任意报告分子。 在解决了几个技术问题后，原型探针已经构建完成。 为了避免破坏离子载体的选择性，必须对光纤进行硅烷化。 硅烷化后，根据钙选择性和质子选择性离子载体的测量，离子载体的选择性没有变化。 将激发光耦合到光纤中需要以高频率 (20 kHz) 振动光纤加载端，以确保均匀传输光学模式并减少激光散斑。 该显微镜配备了基于荧光素的滤光片，可以传输蓝色激光（氩离子激光）并检测绿色发射光。 综合平台 当新技术与并行运行的现有技术相结合并提供相互佐证时，新技术会更普遍地被接受。 BRC 已经维护了组合的电化学和电生理学平台，后者包括单个微电极、电压、电流和膜片钳。