DIRECT DIGITAL IMAGING SYSTEMS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 开发用于中压电子显微镜(IVEM)的数字成像系统是本核心项目的目标。在过去的一年里，我们为Tietz和Gatan相机功能编写了一套完整的插件式通信模块，可以通过Web访问。这些相机现在被用于开发新的光学切片功能和通过网络进行断层扫描的常规程序。自那以后，我们开发了新的自动化优等度算法和镶嵌技术，以使用这些系统获取大范围图像。我们依靠内部基于Java的软件，使用通信模块集成来自多个制造商的多个系统。在目前的资助期内，我们努力完成实验室仪器现代化的整合。我们已经开始将所有显微镜、舞台和相机控制整合到一个控制包中。这一整合是通过与科罗拉多州博尔德的David Mastronarde合作进行的，以生产在我们所有IVEM上运行的通用版本的SerialEM断层扫描软件。特别是，我们在扩展SerialEM开源解决方案以适应我们定制的电荷耦合器件和直接电子探测器系统的控制方面取得了进展。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Developing digital imaging systems for intermediate voltage electron microscope (IVEM) is the objective of this core project. In the previous year we have written a complete set of plug-in communication modules for the Tietz and Gatan camera functions that will enable accessibility through the Web. These cameras are now being used in the development of new optical sectioning functions and tomography routines via the Web. We have since developed new automated eucentricity algorithms and mosaicing techniques to acquire large field images with these systems. We rely on in-house JAVA based software to integrate multiple systems from multiple manufacturers using communication modules. In this current funding period, we have strived to complete the integration of the modernization of the instruments in the laboratory. We have begun the process of combining all of the microscope, stage and camera control into a single control package. This integration being undertaken through a collaboration with David Mastronarde at Boulder Colorado to produce a generalized version of SerialEM tomography software that runs on all of our IVEMs. In particular, we have made progress to extend the SerialEM open source solution to accommodate the control of our custom CCD and Direct Electron Detector systems.