CORE--CRYSTALLOGRAPHY

核心--晶体学

• 批准号: 6316626
• 负责人: NGUYEN-HUU H XUONG
• 金额: $ 14.1万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-12-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6316626
• 关键词: X ray crystallography; binding proteins; bioimaging /biomedical imaging; biomedical facility; protein kinase; protein kinase A; protein kinase C

Protein crystallography is an extremely powerful tool to determine the three-dimensional, high resolution structures of proteins. However, protein crystallography is technically demanding, and requires expensive instrumentation and highly skilled and experienced personnel. The purpose of Core B is to provide this expertise and make it possible for all members of the Program Project to apply these techniques in their program related research. Personnel associated with Core B have considerable experience in all facets of protein crystallography for the last 25 years. He was the inventor of the "Multiwire Area Detectors" which revolutionized the data collection method for protein crystallography. He has also run an NIH Resource Center for protein crystallography for 10 years. Dr. Madhusudan, the scientific director, has used protein crystallography for many years to successfully solve many protein structures. His expertise is specifically in the area of protein kinase. Mr. Chris Nielsen is an experienced programmer who has worked on data collection and analysis for almost 20 years. We intend to operate the facility as a state-of-the-art Core to serve laboratory program members. Three crystallographic projects are proposed. (1) High resolution structures of the dual specific A-Kinase Anchoring Proteins, the D-AKAP's, that interaction with both RI and RII subunits of PKA (Project 1: Taylor) will be solved both in the absence and presence of the dimerization/docking domain or the full length RI and RII subunits. (2) High resolution structures of the PKC kinase domain, with its's carboxyl terminal docking domain in the dephosphorylated or phosphorylated form (Project 3:Newton (3). The structure of the newly discovered AKAP in gravin will be solved alone and as a complex with the dimerization/docking domain of RIIa and RIIb. (Project 2:Scott and Project 4:Jennings).

蛋白质晶体学是确定蛋白质的极其强大的工具 蛋白质的三维、高分辨率结构。然而， 蛋白质晶体学技术要求高，并且需要昂贵的费用 仪器仪表和技术精湛、经验丰富的人员。目的 核心 B 的目的是提供这种专业知识，并使所有人都能 项目成员将这些技术应用到他们的项目中 相关研究。与核心 B 相关的人员拥有相当多的 过去 25 年在蛋白质晶体学的各个方面拥有丰富的经验。 他是“多线区域探测器”的发明者，彻底改变了 蛋白质晶体学的数据收集方法。他还经营过一家 NIH 蛋白质晶体学资源中心 10 年。博士。 科学主任 Madhusudan 使用蛋白质晶体学进行了 多年来成功解决了许多蛋白质结构。他的专长是 特别是在蛋白激酶领域。克里斯·尼尔森先生是 经验丰富的程序员，曾从事数据收集和分析工作 快20年了。我们打算将该设施作为最先进的设施来运营 服务实验室计划成员的核心。 提出了三个晶体学项目。 (1) 高分辨率 双特异性 A-激酶锚定蛋白 D-AKAP 的结构， 与 PKA 的 RI 和 RII 亚基相互作用（项目 1：Taylor） 在不存在和存在的情况下都将得到解决 二聚化/对接结构域或全长 RI 和 RII 亚基。 (2) PKC 激酶结构域及其羧基的高分辨率结构 去磷酸化或磷酸化形式的末端对接结构域 （项目3：牛顿（3）。新发现的AKAP的结构 gravin 将单独求解，并作为一个复合体与 RIIa 和 RIIb 的二聚化/对接结构域。 （项目 2：斯科特和项目 4：詹宁斯）。