CORE--CRYSTALLOGRAPHY

核心--晶体学

• 批准号: 6105864
• 负责人: NGUYEN-HUU H XUONG
• 金额: $ 14.1万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-12-15 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6105864
• 关键词: X ray crystallography; binding proteins; bioimaging /biomedical imaging; biomedical facility; protein kinase; protein kinase A; protein kinase C

Protein crystallography is an extremely powerful tool to determine the three-dimensional, high resolution structures of proteins. However, protein crystallography is technically demanding, and requires expensive instrumentation and highly skilled and experienced personnel. The purpose of Core B is to provide this expertise and make it possible for all members of the Program Project to apply these techniques in their program related research. Personnel associated with Core B have considerable experience in all facets of protein crystallography for the last 25 years. He was the inventor of the "Multiwire Area Detectors" which revolutionized the data collection method for protein crystallography. He has also run an NIH Resource Center for protein crystallography for 10 years. Dr. Madhusudan, the scientific director, has used protein crystallography for many years to successfully solve many protein structures. His expertise is specifically in the area of protein kinase. Mr. Chris Nielsen is an experienced programmer who has worked on data collection and analysis for almost 20 years. We intend to operate the facility as a state-of-the-art Core to serve laboratory program members. Three crystallographic projects are proposed. (1) High resolution structures of the dual specific A-Kinase Anchoring Proteins, the D-AKAP's, that interaction with both RI and RII subunits of PKA (Project 1: Taylor) will be solved both in the absence and presence of the dimerization/docking domain or the full length RI and RII subunits. (2) High resolution structures of the PKC kinase domain, with its's carboxyl terminal docking domain in the dephosphorylated or phosphorylated form (Project 3:Newton (3). The structure of the newly discovered AKAP in gravin will be solved alone and as a complex with the dimerization/docking domain of RIIa and RIIb. (Project 2:Scott and Project 4:Jennings).

蛋白质结晶学是一种非常强大的工具来确定 蛋白质的三维高分辨率结构。然而， 蛋白质结晶学在技术上要求很高，而且需要昂贵的成本。 仪器仪表和高技能、有经验的人员。目的 核心B的核心是提供这种专业知识，并使所有人都有可能 计划项目的成员在他们的计划中应用这些技术 相关研究。与核心B相关的人员有相当大的 在过去的25年里，在蛋白质结晶学的各个方面都有经验。 他是“多线区域探测器”的发明者，它彻底改变了 蛋白质结晶学的数据采集方法。他还经营着一家 美国国立卫生研究院蛋白质结晶学资源中心工作10年。Dr。 科学主任马多苏丹利用蛋白质结晶学研究 多年来成功地解决了许多蛋白质结构问题。他的专长是 特别是在蛋白激酶这一领域。克里斯·尼尔森先生是一位 从事数据收集和分析工作的经验丰富的程序员 差不多20年了。我们打算把这个设施作为最先进的设施来运营 核心服务于实验室项目成员。 提出了三个结晶学方案。(1)高分辨率 双特异性A-激酶锚定蛋白D-AKAP的结构， 与PKA的RI和RII亚基的相互作用(项目1：泰勒) 将在缺席和在场的情况下得到解决 二聚/对接结构域或全长RI和RII亚基。(2) 蛋白激酶C结构域及其羧基的高分辨结构 去磷酸化或磷酸化形式的末端对接结构域 (项目3：牛顿(3)。新发现的AKAP的结构 格雷文将单独解决，并作为一个复杂的 RIIA和RIIB的二聚化/对接结构域。(项目2：斯科特和项目 4：詹宁斯)。