DIRECT DIGITAL IMAGING SYSTEMS

直接数字成像系统

• 批准号: 7957587
• 负责人: NGUYEN-HUU H XUONG
• 金额: $ 20.26万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-15 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957587
• 关键词: Algorithms; Collaborations; Colorado; Communication; Computer Retrieval of Information on Scientific Projects Database; Computer software; Custom; Development; Electron Microscope; Electrons; Funding; Grant; Housing; Image; Image Analysis; Institution; Internet; Java; Laboratories; Manufacturer Name; Microscope; Modernization; Optics; Plug-in; Process; Research; Research Personnel; Resources; Running; Solutions; Source; Staging; System; Techniques; United States National Institutes of Health; Writing; base; detector; digital; digital imaging; instrument; open source; tomography; voltage

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Developing digital imaging systems for intermediate voltage electron microscope (IVEM) is the objective of this core project. In the previous year we have written a complete set of plug-in communication modules for the Tietz and Gatan camera functions that will enable accessibility through the Web. These cameras are now being used in the development of new optical sectioning functions and tomography routines via the Web. We have since developed new automated eucentricity algorithms and mosaicing techniques to acquire large field images with these systems. We rely on in-house JAVA based software to integrate multiple systems from multiple manufacturers using communication modules. In this current funding period, we have strived to complete the integration of the modernization of the instruments in the laboratory. We have begun the process of combining all of the microscope, stage and camera control into a single control package. This integration being undertaken through a collaboration with David Mastronarde at Boulder Colorado to produce a generalized version of SerialEM tomography software that runs on all of our IVEMs. In particular, we have made progress to extend the SerialEM open source solution to accommodate the control of our custom CCD and Direct Electron Detector systems.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本核心项目的目标是研制中压电子显微镜数字成像系统。在过去的一年里，我们已经为Tietz和Gatan相机功能编写了一套完整的插件通信模块，这些模块将通过Web实现可访问性。这些摄像机现在正用于开发新的光学切片功能和通过网络进行断层扫描。从那时起，我们开发了新的自动eucentricity算法和镶嵌技术，以获得大视场图像与这些系统。我们依靠内部基于JAVA的软件，使用通信模块集成来自多个制造商的多个系统。在目前的资助期间，我们努力完成实验室仪器现代化的整合。 我们已经开始将所有的显微镜、载物台和摄像机控制组合到一个控制包中。这种集成是通过与科罗拉多博尔德的大卫Mastronarde合作进行的，以产生在我们所有的IVEM上运行的SerialEM断层扫描软件的通用版本。 特别是，我们在扩展SerialEM开源解决方案方面取得了进展，以适应我们定制的CCD和直接电子探测器系统的控制。