DATA COLLECTION ON CRYSTALS OF REGULATORY SUBUNIT OF PKA

PKA 调节亚基晶体的数据收集

• 批准号: 6119425
• 负责人: NGUYEN-HUU H XUONG
• 金额: --
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2000-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6119425
• 关键词: biomaterials; proteins

In the molecular scheme of living organisms, adenosine 3'-5' monophosphate (cyclic AMP or cAMP) has been a universal second messenger. In eukaryotic cells, the primary receptor for cAMP are regulatory subunits of cAMP-dependent protein kinase. The crystal structure of a 1-91 deletion mutant of the type Ia regulatory subunit was refined to 2.8 E resolution using data from 7-1 beam line of SSRL. Each of the two tandem cAMP binding domains provides an extensive network of hydrogen bonds that buries the cyclic phosphate and the ribose between the two _ -strands that are linked by a short ?-helix. Each adenine base stacks against an aromatic ring that lies outside the _-barrel. This structure provides a molecular basis of understanding how cAMP binds cooperatively to its receptor protein, thus mediating activation of the kinase. Since the determination of the deletion mutant structure, crystals of the wild-type Ia regulatory dimer have been obtained. A low resolution 4.5 E data set from these crystals was collected in the laboratory source. We have yet to succeed in our attempts to get better data from the synchrotron source.

在生物体的分子结构中，腺苷3‘-5’