CORE--CRYSTALLOGRAPHY

蛋白质晶体学是一个非常强大的工具， 蛋白质的三维高分辨率结构。然而，在这方面， 蛋白质晶体学在技术上要求很高， 仪器和高度熟练和经验丰富的人员。目的 核心B的目标是提供这种专业知识， 计划项目的成员在他们的计划中应用这些技术 相关研究。与核心B相关的人员有相当多的 在过去的25年里，他在蛋白质晶体学的各个方面都有丰富的经验。 他是“多线区域探测器”的发明者， 蛋白质晶体学的数据收集方法。他还经营着一家 在NIH蛋白质晶体学资源中心工作了10年。博士 科学总监Madhusudan利用蛋白质晶体学， 多年来，成功地解决了许多蛋白质结构。他的专长是 特别是在蛋白激酶领域。Chris Nielsen是一位 经验丰富的程序员，曾从事数据收集和分析工作， 快20年了我们打算把这个设施作为最先进的 核心服务于实验室计划成员。 提出了三个晶体学方案。(1)高分辨率 双特异性A-激酶辅助蛋白，D-AKAP， 与PKA的RI和RII亚基相互作用（项目1：Taylor） 将在缺席和在场的情况下得到解决 二聚化/对接结构域或全长RI和RII亚基。（二） PKC激酶结构域及其羧基的高分辨率结构 脱磷酸化或磷酸化形式的末端对接域 （项目3：牛顿（3）。新发现的AKAP的结构 Gravin将被单独解决，并作为一个复杂的 RIIa和RIIb的二聚化/对接结构域。（项目2：斯科特和项目） 4：Jennings）.

Protein crystallography is an extremely powerful tool to determine the three-dimensional, high resolution structures of proteins. However, protein crystallography is technically demanding, and requires expensive instrumentation and highly skilled and experienced personnel. The purpose of Core B is to provide this expertise and make it possible for all members of the Program Project to apply these techniques in their program related research. Personnel associated with Core B have considerable experience in all facets of protein crystallography for the last 25 years. He was the inventor of the "Multiwire Area Detectors" which revolutionized the data collection method for protein crystallography. He has also run an NIH Resource Center for protein crystallography for 10 years. Dr. Madhusudan, the scientific director, has used protein crystallography for many years to successfully solve many protein structures. His expertise is specifically in the area of protein kinase. Mr. Chris Nielsen is an experienced programmer who has worked on data collection and analysis for almost 20 years. We intend to operate the facility as a state-of-the-art Core to serve laboratory program members. Three crystallographic projects are proposed. (1) High resolution structures of the dual specific A-Kinase Anchoring Proteins, the D-AKAP's, that interaction with both RI and RII subunits of PKA (Project 1: Taylor) will be solved both in the absence and presence of the dimerization/docking domain or the full length RI and RII subunits. (2) High resolution structures of the PKC kinase domain, with its's carboxyl terminal docking domain in the dephosphorylated or phosphorylated form (Project 3:Newton (3). The structure of the newly discovered AKAP in gravin will be solved alone and as a complex with the dimerization/docking domain of RIIa and RIIb. (Project 2:Scott and Project 4:Jennings).