HISTOLOGY

组织学

• 批准号: 7515263
• 负责人: DENNIS CARSON
• 金额: $ 13.23万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7515263
• 关键词: Antibodies; Attention; Biological Assay; Cancer Center; Client; Confocal Microscopy; Consultations; Data; Enzyme-Linked Immunosorbent Assay; Equipment; Flow Cytometry; Freezing; Frozen Sections; Future; Genes; Hematoxylin and Eosin Staining Method; Hemorrhage; Histocytochemistry; Histologic; Histology; Histopathology; Human; Human Resources; Immunohistochemistry; Individual; Inflammation; Investigation; Malignant Neoplasms; Methods; Microtome - medical device; Morphology; Mus; Necrosis; Numbers; Organ; Paraffin; Pathologist; Pathology; Polymerase Chain Reaction; Process; Proteins; Quality Control; Rate; Reagent; Research Personnel; Resource Sharing; Resources; Role; Sampling; Services; Staining method; Stains; Time; Tissue Embedding; Tissue Extracts; Tissues; Training; Translating; Western Blotting; base; cell type; cryostat; experience; human disease; member; mouse model; programs; research study; single cell analysis; tumor; volunteer

Histology and Immunohistochemistry The Histology and Immunohistochemistry Resource is crucial for providing the prerequisite morphologic data on any sample of organ or tissue, prior to the use of the excised tissue in different analytical assays. Histological examination of tissue thus establishes the absence of abnormal pathology, such as inflammation, necrosis, hemorrhage or tumor, prior to using the extracts of the tissue in various experiments. Scientific investigation of the role of particular genes in cancer is very often dependent on the ability to translate observations obtained from methods such as enzyme linked immunosorbent assays, polymerase chain reaction or even Northern, Southern, Western blots, into identifying the cell types of expression in tissue sections. The analysis of single cells by flow cytometry, or the analysis of tissue sections with confocal microscopy is facilitated by the use of confirmatory histological methods. Very often, it is the expression of gene products in a particular tissue that allows the investigator to deduce the significance of its presence to its function. The results obtained from analysis of tissue are also critical in the planning of future directions of projects. The minimal recharge rates of this popular resource also permits Cancer Center members to examine tissues obtained from various mouse models of cancer. Although mouse histopathology is similar to human histopathology, there are numerous differences, which can confound accurate interpretation. Thus the readily available dne-on-one consultation with the resource leader, a board certified pathologist, experienced with interpretation of mouse models of human disease, is a critical asset of this resource.

组织学和免疫组织化学 组织学和免疫组织化学资源是提供必要的形态数据的关键 在将切除的组织用于不同的分析分析之前，对任何器官或组织样本进行检测。 因此，组织的组织学检查确定没有异常病理，例如 炎症、坏死、出血或肿瘤，在各种实验中使用组织提取物之前。 对特定基因在癌症中的作用的科学研究往往依赖于 从酶联免疫吸附试验、聚合酶 连锁反应甚至Northern、Southern、Western印迹，以鉴定细胞类型在 组织切片。单细胞的流式细胞术分析，或组织切片的共聚焦分析 通过使用验证性的组织学方法，显微镜检查变得容易。很多时候，这是一种表达 特定组织中的基因产物，使研究人员能够推断其存在对 它的功能。从组织分析中获得的结果对未来的方向规划也是至关重要的。 项目。 这种受欢迎的资源的最低充电率也允许癌症中心成员检查 从各种癌症小鼠模型中获得的组织。尽管小鼠的组织病理学与人类相似 组织病理学上，有许多不同之处，这可能会混淆准确的解释。因此， 随时可以与资源负责人进行一对一的咨询，他是委员会认证的病理学家，经验丰富 用来解释人类疾病的小鼠模型，是这一资源的关键资产。