CELL PURIFICATION AND ANALYSIS LABORATORY

一个共享的“细胞纯化和分析实验室”被提议作为该计划项目的核心 核心的主要责任有两个方面。首先，这个核心负责 处理人和鼠的造血组织，以分离和纯化表型确定的 使用免疫磁性选择、富集或去除程序的造血细胞群体 和流式细胞术细胞分选。第二，核心将协调、执行、分析和协助解释 流式细胞术分析造血和非造血未操纵或 用于或产生于所提出的研究的转导细胞。在履行这些基本职责时，该核心将为项目的所有子项目的实施发挥基本作用。期间 在上一个供资期间，这一核心资源为联合国所有项目的执行提供了宝贵的援助， 直接受益于该核心提供的服务的出版物清单表明，该计划是有效的。 Tie流式细胞术资源设施（FCRF）将容纳拟议的核心，配备齐全， 用于执行复杂的流式细胞术程序的最先进仪器， 是印第安纳州大学癌症中心的核心单位。目前，FCRF 位于印第安纳州癌症研究所的一楼，英尺实验室与 毗邻办公室/电脑室（170平方英尺）。 联邦储备基金的中心位置 极大地方便了该核心的所有主要研究人员的实验室的访问， 程序项目。 共享的细胞纯化和分析实验室将为项目参与者提供 经济地使用设备和空间以及进行所需的必要细胞群， 他们的研究。此外，这样一个核心实验室将被定位为分享和传播科学知识， 从该计划的不同项目中收集的信息。

A shared "Cell Purification and Analysis Laboratory" is proposed as this Core for the program project The primary responsibilities of the Core are two fold. First, this core is charged with the responsibility of processing human and murine hematopoietic tissues in order to isolate and purify phenotypically defined populations of hematopoietic cells using immunomagnetic selection, enrichment, or depletion procedures and flow cytometric cell sorting. Second, the core will coordinate, perform, analyze, and assist in interpreting the results of flow cytometric analyses of hematopoietic and non-hematopoietic unmariipulated or transduced cells used for or resulting from the proposed studies. In performing these essential duties, this Core will serve a fundamental function for the conduct of all subprojects of the program project. During the previous funding period, this core provided valuable assistance for the performance of all projects of the program as is evident by the list of publications benefiting directly from services provided by this Core. Tie flow cytometry resource facility (FCRF), which will house the proposed core, is fully equipped with state of the art instrumentation for the performance of sophisticated flow cytometry procedures required by the proposed studies and is a core unit of the Indiana University Cancer Center. The FCRF is currently located on the first floor of the Indiana Cancer Research Institute in a separate 850 sq. ft. laboratory with an adjoining office/computer room (170 square feet). This central location of the FCRF greatly facilitates the accessibility of this core to the laboratories of all the principal investigators of the program project. The shared cell purification and analysis laboratory will provide for participants in the program project an economical use of equipment and space as well as necessary cell populations required for the conduct of their research. In addition, such a core laboratory will be positioned to share and disseminate scientific information collected from the different projects of this program.