Impact of host iron status on growth and viability of erythrocytic stages of Plas

说明(申请人提供)：缺铁是世界上最常见的孕妇和儿童微量营养素缺乏症之一，它导致围产期死亡、发病率和发育迟缓的风险增加。最近的证据表明，缺铁可以预防疟疾。因此，在世界疟疾流行地区普遍补充剂的智慧受到了密切关注。这项应用将试图确定人类宿主的铁状态在体外影响恶性疟原虫寄生虫的机制。具体地说，我们将确定在补充铁前后，严重缺铁者的红细胞和/或血清中恶性疟原虫的生长、成熟和侵袭率是否降低。这一结果将有助于在疟疾流行地区制定以证据为基础的铁补充政策。 与公共卫生相关：缺铁影响着5亿多人，包括世界疟疾肆虐地区的许多孕妇和儿童。有人提出，缺铁可以预防疟疾，也有人对疟疾流行国家的普遍补铁计划带来的风险提出了质疑。在拟议的项目中，我们将确定疟疾寄生虫的生长和发育是否在体外受到宿主铁状态的影响。我们将使用严重缺铁性贫血患者的红细胞和血清来非常系统地看待这个问题。这项研究将提供支持或反对缺铁可能影响疟疾寄生虫的可能机制的信息。最终，这些信息将为确定疟疾流行地区的补铁政策提供更多证据。

DESCRIPTION (provided by applicant): Iron deficiency is one of the most common micronutrient deficiencies in the world in pregnant women and children and it causes increased risk of perinatal mortality, morbidity and developmental delay. Recent evidence has suggested that iron deficiency may protect against malaria. Thus, the wisdom of universal supplementation in areas of the world where malaria is endemic has come under scrutiny. This application will attempt to identify the mechanism by which the human host's iron status affects the P. falciparum parasite in vitro. Specifically, we will determine if the growth, maturation and invasion rate of P. falciparum parasites is reduced in red blood cells and/or serum from severely iron deficient individuals before and after iron supplementation. The results will contribute to efforts to develop evidence-based iron supplementation policies in malaria-endemic areas. PUBLIC HEALTH RELEVANCE: Iron deficiency affects over 500 million people including many pregnant women and children from areas of the world that are plagued by malaria. It has been suggested that iron deficiency protects against malaria and questions have been raised about the risks associated with the universal iron supplementation programs in countries where malaria is endemic. In the proposed project, we will determine whether the growth and development of the malaria parasite is impacted by the host iron status in vitro. We will use RBC and sera from patients with severe iron deficiency anemia to look at this question in a very systematic way. This study will provide information for or against a possible mechanism by which iron deficiency may affect the malaria parasite. Ultimately, this information will provide additional evidence for the determination of policy on iron supplementation in malaria endemic regions.