SULFATED STEROIDS AS NATURAL LIGANDS OF MOUSE PHEROMONE-SENSING NEURONS

硫酸类固醇作为小鼠信息素感知神经元的天然配体

• 批准号: 7954013
• 负责人: FRANCESCO NODARI
• 金额: $ 0.57万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-02-01 至 2010-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954013
• 关键词: Afferent Neurons; Charge; Computer Retrieval of Information on Scientific Projects Database; Cues; Female; Funding; Grant; Hydrophobicity; Institution; Ligands; Mass Spectrum Analysis; Molecular; Molecular Weight; Mus; Nature; Neurons; Odors; Pheromone; Property; Research; Research Personnel; Resources; Social status; Source; United States National Institutes of Health; Urine; biomedical resource; sex; social; steroid sulfate

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Among mice, pheromones and other social odor cues convey information about sex, social status, and identity; however, the molecular nature of these cues is essentially unknown. To identify these cues, we screened chromatographic fractions of female mouse urine for their ability to cause reproducible firing rate increases in the pheromone-detecting vomeronasal sensory neurons (VSNs) using multielectrode array (MEA) recording. Active compounds were found to be remarkably homogenous in their basic properties, with most being of low molecular weight, moderate hydrophobicity, low volatility, and possessing a negative electric charge.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在小鼠中，信息素和其他社会气味线索传达有关性别，社会地位和身份的信息;然而，这些线索的分子本质本质基本上是未知的。为了确定这些线索，我们筛选了雌性小鼠尿液的色谱组分，其能力，导致可重复的发射率增加的信息素检测犁鼻感觉神经元（VSN）使用多电极阵列（MEA）记录。发现活性化合物在其基本性质上是非常均匀的，大多数具有低分子量、中等疏水性、低挥发性和负电荷。