TURNOVER RATE OF PRP OLIGOMERS IN THE BRAIN

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 Prion是一种具有感染性的蛋白质颗粒，会导致致命的神经退行性疾病。PrP是感染颗粒中的主要蛋白质，是一种糖脂膜锚定蛋白。去除糖脂会导致大脑内形成大量具有感染性的聚集体。利用氮同位素掺入，我想研究这种可溶性PrP异构体形成的聚集体内的周转率。 利用纯化蛋白质的同位素分布随时间的变化，我将能够计算出聚集体的周转率。质谱仪将协助测定这些蛋白质的同位素比率。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Prions are infectious proteinaceous particles which cause fatal neurodegenerative diseases. PrP the predominant protein within the infectious particles is a glycolipid membrane anchored protein. Removing the glycolipid induces large amounts of infectious aggregates to form within the brain. Using nitrogen isotope incorporation I want to investigate the rate of turnover within the aggregates formed from this soluble PrP isoform. Using the change in isotopic distribution of purified protein over time I will be able to calculate a turnover rate for the aggregate. The mass spec facility will assist in the determination of the isotope ratio for these proteins.