TURNOVER RATE OF PRP OLIGOMERS IN THE BRAIN

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。所列机构为 中心，不一定是研究者的机构。 朊病毒是一种可引起致命性神经退行性疾病的感染性蛋白质颗粒。 感染颗粒内的主要蛋白质PrP是糖脂膜锚定蛋白。 去除糖脂会诱导大量感染性聚集物在脑内形成。 使用氮同位素掺入，我想调查的周转率从这个可溶性PrP亚型形成的聚集体内。 利用纯化蛋白质同位素分布随时间的变化，我将能够计算聚集体的周转率。 质谱设备将有助于确定这些蛋白质的同位素比。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Prions are infectious proteinaceous particles which cause fatal neurodegenerative diseases. PrP the predominant protein within the infectious particles is a glycolipid membrane anchored protein. Removing the glycolipid induces large amounts of infectious aggregates to form within the brain. Using nitrogen isotope incorporation I want to investigate the rate of turnover within the aggregates formed from this soluble PrP isoform. Using the change in isotopic distribution of purified protein over time I will be able to calculate a turnover rate for the aggregate. The mass spec facility will assist in the determination of the isotope ratio for these proteins.