TURNOVER RATE OF PRP OLIGOMERS IN THE BRAIN
TURNOVER RATE OF PRP OLIGOMERS IN THE BRAIN
批准号:
7957429
负责人:
STANLEY B PRUSINER
金额:
$0.1万
依托单位国家:
美国
项目类别:
财政年份:
2009
资助国家:
美国
项目状态:
已结题
起止时间:
2009-06-01 至 2010-05-31
关键词:
BrainComputer Retrieval of Information on Scientific Projects DatabaseFundingGlycolipidsGrantInstitutionIsotopesMass Spectrum AnalysisMembraneNeurodegenerative DisordersNitrogen IsotopesPrPPrionsProtein IsoformsProteinsResearchResearch PersonnelResourcesSourceTimeUnited States National Institutes of Healthisotope incorporationparticle
中文摘要
这个子项目是许多研究子项目中的一个
由NIH/NCRR资助的中心赠款提供的资源。子项目和
研究者(PI)可能从另一个NIH来源获得主要资金,
因此可以在其他CRISP条目中表示。所列机构为
中心,不一定是研究者的机构。
朊病毒是一种可引起致命性神经退行性疾病的感染性蛋白质颗粒。 感染颗粒内的主要蛋白质PrP是糖脂膜锚定蛋白。 去除糖脂会诱导大量感染性聚集物在脑内形成。 使用氮同位素掺入,我想调查的周转率从这个可溶性PrP亚型形成的聚集体内。
利用纯化蛋白质同位素分布随时间的变化,我将能够计算聚集体的周转率。 质谱设备将有助于确定这些蛋白质的同位素比。
英文摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
Prions are infectious proteinaceous particles which cause fatal neurodegenerative diseases. PrP the predominant protein within the infectious particles is a glycolipid membrane anchored protein. Removing the glycolipid induces large amounts of infectious aggregates to form within the brain. Using nitrogen isotope incorporation I want to investigate the rate of turnover within the aggregates formed from this soluble PrP isoform.
Using the change in isotopic distribution of purified protein over time I will be able to calculate a turnover rate for the aggregate. The mass spec facility will assist in the determination of the isotope ratio for these proteins.
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STRUCTURAL CHARACTERIZATION OF PRION PROTEINS
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批准号:8363722
-
项目类别:
-
资助金额:$0.0万
-
财政年份:2011
-
负责人:STANLEY B PRUSINER
-
依托单位:
IDENTIFICATION OF LIPIDS ASSOCIATED WITH PRIONS
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批准号:8365561
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项目类别:
-
资助金额:$1.08万
-
财政年份:2011
-
负责人:STANLEY B PRUSINER
-
依托单位:
BIOCHEMICAL AND BIOPHYSICAL CHARACTERIZATION OF PRION PROTEIN 2D CRYSTALS
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批准号:8363780
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项目类别:
-
资助金额:$0.04万
-
财政年份:2011
-
负责人:STANLEY B PRUSINER
-
依托单位:
TURNOVER RATE OF PRP OLIGOMERS IN THE BRAIN
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批准号:8363794
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项目类别:
-
资助金额:$3.32万
-
财政年份:2011
-
负责人:STANLEY B PRUSINER
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依托单位:
DYNAMIC SILAC FOR THE STUDY OF PRION PROPAGATION
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批准号:8363818
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项目类别:
-
资助金额:$0.56万
-
财政年份:2011
-
负责人:STANLEY B PRUSINER
-
依托单位:
TURNOVER RATE OF PRP OLIGOMERS IN THE BRAIN
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批准号:8169789
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项目类别:
-
资助金额:$0.18万
-
财政年份:2010
-
负责人:STANLEY B PRUSINER
-
依托单位:
DYNAMIC SILAC FOR THE STUDY OF PRION PROPAGATION
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批准号:8169814
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项目类别:
-
资助金额:$0.35万
-
财政年份:2010
-
负责人:STANLEY B PRUSINER
-
依托单位:
BIOCHEMICAL AND BIOPHYSICAL CHARACTERIZATION OF PRION PROTEIN 2D CRYSTALS
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批准号:8169775
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项目类别:
-
资助金额:$0.35万
-
财政年份:2010
-
负责人:STANLEY B PRUSINER
-
依托单位:
IDENTIFICATION OF LIPIDS ASSOCIATED WITH PRIONS
-
批准号:8170935
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项目类别:
-
资助金额:$1.91万
-
财政年份:2010
-
负责人:STANLEY B PRUSINER
-
依托单位:
STRUCTURAL CHARACTERIZATION OF PRION PROTEINS
-
批准号:8169717
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项目类别:
-
资助金额:$0.18万
-
财政年份:2010
-
负责人:STANLEY B PRUSINER
-
依托单位:
IDENTIFICATION OF LIPIDS ASSOCIATED WITH PRIONS
-
批准号:7955978
-
项目类别:
-
资助金额:$0.58万
-
财政年份:2009
-
负责人:STANLEY B PRUSINER
-
依托单位:
STRUCTURAL CHARACTERIZATION OF PRION PROTEINS
-
批准号:7957354
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项目类别:
-
资助金额:$0.02万
-
财政年份:2009
-
负责人:STANLEY B PRUSINER
-
依托单位:
ADMINISTRATION
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批准号:7638108
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项目类别:
-
资助金额:$9.36万
-
财政年份:2009
-
负责人:STANLEY B PRUSINER
-
依托单位:
BIOCHEMICAL AND BIOPHYSICAL CHARACTERIZATION OF PRION PROTEIN 2D CRYSTALS
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批准号:7957413
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项目类别:
-
资助金额:$0.01万
-
财政年份:2009
-
负责人:STANLEY B PRUSINER
-
依托单位:
ANIMALS
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批准号:7638112
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项目类别:
-
资助金额:$28.67万
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财政年份:2009
-
负责人:STANLEY B PRUSINER
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依托单位:
Degenerative and Dementing Diseases of Aging
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批准号:7908094
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项目类别:
-
资助金额:$10.0万
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财政年份:2009
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负责人:STANLEY B PRUSINER
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依托单位:
INVESTIGATIONS OF HUMAN PRION DISEASE
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批准号:7638103
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项目类别:
-
资助金额:$33.16万
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财政年份:2009
-
负责人:STANLEY B PRUSINER
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依托单位:
Towards Therapeutics for Neurodegenerative Diseases
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批准号:8022864
-
项目类别:
-
资助金额:$59.59万
-
财政年份:2008
-
负责人:STANLEY B PRUSINER
-
依托单位:
Towards Therapeutics for Neurodegenerative Diseases
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批准号:8411612
-
项目类别:
-
资助金额:$59.25万
-
财政年份:2008
-
负责人:STANLEY B PRUSINER
-
依托单位:
Towards Therapeutics for Neurodegenerative Diseases
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批准号:8897681
-
项目类别:
-
资助金额:$6.82万
-
财政年份:2008
-
负责人:STANLEY B PRUSINER
-
依托单位: