IDENTIFICATION OF LIPIDS ASSOCIATED WITH PRIONS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们已经开发了纯化朊病毒的条件，用于结构分析，但这些制剂仍然含有与朊病毒蛋白质相关的脂质。这些脂质之一是神经节苷脂。与朊病毒蛋白相关的另一类脂质是神经酰胺，其通过薄层色谱法（TLC）进行分配。在TLC板上，朊病毒相关脂质部分中至少有一种额外的组分富集，但不容易识别。在资源，LC/MS/MS被用来确定朊病毒相关脂质池的组成部分。这导致了四十多种脂质的鉴定，并使样品之间的比较成为可能。现在，研究人员已经从不同疾病阶段的动物身上制备了样本，并正在进行分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have developed conditions to purify prions for structural analysis, but these preparations still contain lipids that associate with the prion protein. One of these lipids is a ganglioside. Another class of lipids that associate with the prion protein are ceramides, which were assigned by thin layer chromatography (TLC). At least one additional component was enriched in the prion-associated lipid fraction on the TLC plate, but it could not easily be identified. At the Resource, LC/MS/MS is being used to identify the components of the prion-associated lipid pool. This has resulted in the identification of more than forty lipids, and has enabled comparisons between the samples. Now the investigators have prepared samples from animals with different stages of disease and these are being analyzed.