IDENTIFICATION OF LIPIDS ASSOCIATED WITH PRIONS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们已经为结构分析开发了纯化普鲁恩的条件，但这些制剂仍然含有与普恩蛋白相关的脂类。其中一种脂质是神经节苷脂。另一类与Prion蛋白相关的脂类是神经酰胺，薄层色谱(TLC)对其进行了鉴定。至少有一种额外的成分在薄层色谱板上的Prion相关的脂组分中被浓缩，但它不容易被识别。在该资源中心，LC/MS/MS正被用来鉴定与Prion相关的类脂池的成分。这导致了40多种脂类的鉴定，并使样品之间的比较成为可能。现在，研究人员已经准备了来自不同疾病阶段的动物的样本，这些样本正在进行分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have developed conditions to purify prions for structural analysis, but these preparations still contain lipids that associate with the prion protein. One of these lipids is a ganglioside. Another class of lipids that associate with the prion protein are ceramides, which were assigned by thin layer chromatography (TLC). At least one additional component was enriched in the prion-associated lipid fraction on the TLC plate, but it could not easily be identified. At the Resource, LC/MS/MS is being used to identify the components of the prion-associated lipid pool. This has resulted in the identification of more than forty lipids, and has enabled comparisons between the samples. Now the investigators have prepared samples from animals with different stages of disease and these are being analyzed.