DYNAMIC SILAC FOR THE STUDY OF PRION PROPAGATION

用于朊病毒传播研究的动态硅酸

• 批准号: 8363818
• 负责人: STANLEY B PRUSINER
• 金额: $ 0.56万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363818
• 关键词: Amino Acids; Cell Culture Techniques; Cell Line; Cells; Cessation of life; Collaborations; Culture Media; Epitopes; Funding; Grant; Infection; Label; Mammals; Mass Spectrum Analysis; Measures; National Center for Research Resources; Nerve Degeneration; Neurons; Principal Investigator; Prions; Protocols documentation; Research; Research Infrastructure; Resources; Source; Techniques; United States National Institutes of Health; cost; instrumentation; novel; protein aggregate; protein structure; transmission process

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Prions are infectious protein aggregates which cause neurodegeneration and death in mammals. Cultured neuronal cells have been used in the study of prions. The study of prion propagation in these cell lines is complicated by an inability to differentiate prions supplied to initiate the infection from new cellular prions. Introducing an novel epitope into cellular prion protein has been used previously to identify prion origin. This technique is problematic because pertubations to protein structure may influence prion transmission. Labeled amino acids in the culture medium would be incorporated into newly synthesized prions. The isotopic label in combination with mass spectroscopy would allow us to identify prions produced in the cell culture. Collaboration with the UCSF Mass Spectrometry Facility would give us access to the instrumentation necessary to measure the extent of isotopic incorporation into the prions we have generated. We have had successful collaborations on similar projects. We envision that this project would use the protocols we have established previously.

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