IDENTIFICATION OF LIPIDS ASSOCIATED WITH PRIONS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 我们已经为结构分析开发了纯化普鲁恩的条件，但这些制剂仍然含有与普恩蛋白相关的脂类。脂质POs中包括神经节苷脂和神经酰胺，最初由薄层色谱(TLC)确定；这些类别的复杂混合物现在已通过串联质谱学确定。在该资源中心，LC/MS/MS正被用来鉴定与Prion相关的类脂池的成分。这导致了100多种脂类的鉴定，并使样品之间的比较成为可能。来自不同疾病阶段的动物的进一步样本正在进行分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We have developed conditions to purify prions for structural analysis, but these preparations still contain lipids that associate with the prion protein. Included in the lipid poos are gangliosides and ceramides, which were initially assigned by thin layer chromatography (TLC); complex mixtures of these classes have now been defined by tandem mass spectrometry. At the Resource, LC/MS/MS is being used to identify the components of the prion-associated lipid pool. This has resulted in the identification of more than one hundred lipids, and has enabled comparisons between the samples. Further samples from animals with different stages of disease are being analyzed.