IDENTIFICATION OF LIPIDS ASSOCIATED WITH PRIONS

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 为子项目列出的总成本可能 表示子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 我们已经开发了纯化朊病毒的条件，用于结构分析，但这些制剂仍然含有与朊病毒蛋白质相关的脂质。包括在脂质poos是神经节苷脂和神经酰胺，这是最初指定的薄层色谱法（TLC），这些类别的复杂混合物，现在已被定义为串联质谱。在资源，LC/MS/MS被用来确定朊病毒相关脂质池的组成部分。这导致了超过一百种脂质的鉴定，并使样品之间的比较成为可能。来自不同疾病阶段动物的进一步样本正在分析中。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We have developed conditions to purify prions for structural analysis, but these preparations still contain lipids that associate with the prion protein. Included in the lipid poos are gangliosides and ceramides, which were initially assigned by thin layer chromatography (TLC); complex mixtures of these classes have now been defined by tandem mass spectrometry. At the Resource, LC/MS/MS is being used to identify the components of the prion-associated lipid pool. This has resulted in the identification of more than one hundred lipids, and has enabled comparisons between the samples. Further samples from animals with different stages of disease are being analyzed.