DYNAMIC SILAC FOR THE STUDY OF PRION PROPAGATION

用于朊病毒传播研究的动态硅酸

• 批准号: 8169814
• 负责人: STANLEY B PRUSINER
• 金额: $ 0.35万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-12 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169814
• 关键词: Amino Acids; Cell Culture Techniques; Cell Line; Cells; Cessation of life; Collaborations; Computer Retrieval of Information on Scientific Projects Database; Culture Media; Epitopes; Funding; Grant; Infection; Institution; Label; Mammals; Mass Spectrum Analysis; Measures; Nerve Degeneration; Neurons; Prions; Protocols documentation; Research; Research Personnel; Resources; Source; Techniques; United States National Institutes of Health; instrumentation; novel; protein aggregate; protein structure; transmission process

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Prions are infectious protein aggregates which cause neurodegeneration and death in mammals. Cultured neuronal cells have been used in the study of prions. The study of prion propagation in these cell lines is complicated by an inability to differentiate prions supplied to initiate the infection from new cellular prions. Introducing an novel epitope into cellular prion protein has been used previously to identify prion origin. This technique is problematic because pertubations to protein structure may influence prion transmission. Labeled amino acids in the culture medium would be incorporated into newly synthesized prions. The isotopic label in combination with mass spectroscopy would allow us to identify prions produced in the cell culture. Collaboration with the UCSF Mass Spectrometry Facility would give us access to the instrumentation necessary to measure the extent of isotopic incorporation into the prions we have generated. We have had successful collaborations on similar projects. We envision that this project would use the protocols we have established previously.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 朊病毒是一种传染性蛋白质聚集体，可导致哺乳动物神经变性和死亡。 培养的神经元细胞已用于朊病毒的研究。 由于无法区分用于启动感染的朊病毒和新细胞朊病毒，对这些细胞系中朊病毒繁殖的研究变得复杂。 先前已使用将新表位引入细胞朊病毒蛋白来鉴定朊病毒起源。这项技术存在问题，因为蛋白质结构的扰动可能会影响朊病毒的传播。培养基中标记的氨基酸将被掺入新合成的朊病毒中。 同位素标记与质谱相结合使我们能够识别细胞培养物中产生的朊病毒。 与加州大学旧金山分校质谱设施的合作将使我们能够使用必要的仪器来测量我们产生的朊病毒的同位素掺入程度。 我们在类似项目上有过成功的合作。 我们预计该项目将使用我们之前建立的协议。