TURNOVER RATE OF PRP OLIGOMERS IN THE BRAIN

该子项目是利用资源的许多研究子项目之一 由NIH/NCRR资助的中心赠款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 为子项目列出的总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 蛋白质聚集体的积累与许多神经退行性疾病有关。 这些蛋白质聚集体的存在是正常蛋白质代谢被破坏的直接证据。 朊病毒是这种效应的主要例子，因为它们是感染性蛋白质颗粒。 虽然朊病毒株已被分离出来，其在孵育时间和病理学上不同，但朊病毒颗粒中没有核酸成分。 感染只需要正常的蛋白质周转被转移到新的聚集体的形成。 了解朊病毒疾病的传播以及这些聚集体如何导致神经变性，需要我们研究朊病毒感染引起的蛋白质周转的变化。 使用氮同位素标记，我们将在蛋白质组范围内测量蛋白质周转，以评估朊病毒疾病期间全球蛋白质周转的扰动。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The accumulation of protein aggregates is associated with many neurodegenerative diseases. The presence of these protein aggregates is direct evidence that normal protein metabolism is disrupted. Prions are a primary example of this effect, as they are infectious proteinaceous particles. Although strains of prions have been isolated which differ in incubation time and pathology, there is no nucleic acid component of the prion particles. Infection requires only that normal protein turnover is diverted to the formation of new aggregates. Understanding the propagation of prion disease and how these aggregates cause neurodegeneration requires that we investigate changes in protein turnover due to prion infection. Using nitrogen isotope labeling we will measure protein turnover on a proteome-wide scale to assess perturbations in global protein turnover during prion disease.