ELECTRONIC NOTEBOOK FOR CRYO-EM

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 Cryo-EM是一个漫长的实验和计算过程，导致最终的3D地图和相关注释。每个步骤都需要不同的专业知识和技能。所有的研究人员都以不同的方式进行实验，以使每个项目都能工作，有些人比其他人更快地完成项目。在我们的中心，每个月都有数十个项目正在进行，每个项目都有不同的技术挑战，处于不同的开发或进展阶段。有些项目只需要几天就可以实现亚纳米分辨率的结构，而另一些项目可能需要数月或数年才能达到相同的水平。很明显，除了各种实验和计算变量之外，调查人员在及时完成项目方面发挥着关键作用。这个核心项目有两个目标。一个目标是将与每个粒子相关的所有元数据存档，从最初嵌入冰中到最终的密度图。第二个目标是开发一种能够合并密度图和相关元数据的档案数据格式。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cryo-EM is a lengthy experimental and computational process, leading to a final 3-D map and related annotations. Each of the steps requires different expertise and skills. All of the investigators perform experiments somewhat differently in order to make each project work, and some manage to complete projects faster than others. In our Center, there are dozens of projects ongoing in any given month, each with different technical challenges and at different stages of development or progress. Some projects take only a few days to achieve a subnanometer resolution structure while others may take months or years to reach the same point. It is obvious that, in addition to various experimental and computational variables, investigators play a critical role in getting the project completed in a timely fashion. There are two aims in this core project. One aim is to archive all the metadata related to each particle from its initial embedding in ice to the final density map. The second aim is to develop an archival data format capable of amalgamating density maps and associated metadata.