ELECTRONIC NOTEBOOK FOR CRYO-EM

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 冷冻-EM是一个漫长的实验和计算过程，导致最终的3-D地图和相关注释。每一步都需要不同的专业知识和技能。所有的研究人员进行实验的方式都略有不同，以使每个项目发挥作用，有些人设法比其他人更快地完成项目。在我们的中心，任何一个月都有数十个正在进行的项目，每个项目都面临着不同的技术挑战，处于不同的开发或进步阶段。一些项目只需几天就能实现亚纳米分辨率的结构，而另一些项目可能需要几个月或几年的时间才能达到相同的水平。显然，除了各种实验和计算变量外，研究人员在及时完成该项目方面发挥着关键作用。这个核心项目有两个目标。其中一个目标是将每个粒子从最初嵌入冰中到最终密度贴图的所有相关元数据存档。第二个目标是开发一种能够合并密度图和相关元数据的档案数据格式。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cryo-EM is a lengthy experimental and computational process, leading to a final 3-D map and related annotations. Each of the steps requires different expertise and skills. All of the investigators perform experiments somewhat differently in order to make each project work, and some manage to complete projects faster than others. In our Center, there are dozens of projects ongoing in any given month, each with different technical challenges and at different stages of development or progress. Some projects take only a few days to achieve a subnanometer resolution structure while others may take months or years to reach the same point. It is obvious that, in addition to various experimental and computational variables, investigators play a critical role in getting the project completed in a timely fashion. There are two aims in this core project. One aim is to archive all the metadata related to each particle from its initial embedding in ice to the final density map. The second aim is to develop an archival data format capable of amalgamating density maps and associated metadata.