ELECTRONIC NOTEBOOK FOR CRYO-EM

用于冷冻电镜的电子笔记本

• 批准号: 8361051
• 负责人: STEVEN J LUDTKE
• 金额: $ 6.13万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361051
• 关键词: 3-Dimensional; Archives; Development; Electron Microscopy; Electronics; Funding; Grant; Ice; Maps; Metadata; National Center for Research Resources; Play; Principal Investigator; Process; Research; Research Infrastructure; Research Personnel; Resolution; Resources; Role; Source; Staging; Structure; United States National Institutes of Health; Work; cost; data format; density; macromolecule; particle; research study; skills

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Cryo-EM is a lengthy experimental and computational process, leading to a final 3-D map and related annotations. Each of the steps requires different expertise and skills. All of the investigators perform experiments somewhat differently in order to make each project work, and some manage to complete projects faster than others. In our Center, there are dozens of projects ongoing in any given month, each with different technical challenges and at different stages of development or progress. Some projects take only a few days to achieve a subnanometer resolution structure while others may take months or years to reach the same point. It is obvious that, in addition to various experimental and computational variables, investigators play a critical role in getting the project completed in a timely fashion. There are two aims in this core project. One aim is to archive all the metadata related to each particle from its initial embedding in ice to the final density map. The second aim is to develop an archival data format capable of amalgamating density maps and associated metadata.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其他NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 Cryo-EM是一个漫长的实验和计算过程，导致最终的3D地图和相关注释。每个步骤都需要不同的专业知识和技能。所有的研究人员都以不同的方式进行实验，以使每个项目都能工作，有些人比其他人更快地完成项目。在我们的中心，每个月都有数十个项目正在进行，每个项目都有不同的技术挑战，处于不同的开发或进展阶段。有些项目只需要几天就可以实现亚纳米分辨率的结构，而另一些项目可能需要数月或数年才能达到相同的水平。很明显，除了各种实验和计算变量之外，调查人员在及时完成项目方面发挥着关键作用。这个核心项目有两个目标。一个目标是将与每个粒子相关的所有元数据存档，从最初嵌入冰中到最终的密度图。第二个目标是开发一种能够合并密度图和相关元数据的档案数据格式。