ELECTRONIC NOTEBOOK FOR CRYO-EM

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 冷冻-EM是一个漫长的实验和计算过程，导致最终的3-D地图和相关注释。每一步都需要不同的专业知识和技能。所有的研究人员进行实验的方式都略有不同，以使每个项目发挥作用，有些人设法比其他人更快地完成项目。在我们的中心，任何一个月都有数十个正在进行的项目，每个项目都面临着不同的技术挑战，处于不同的开发或进步阶段。一些项目只需几天就能实现亚纳米分辨率的结构，而另一些项目可能需要几个月或几年的时间才能达到相同的水平。显然，除了各种实验和计算变量外，研究人员在及时完成该项目方面发挥着关键作用。这个核心项目有两个目标。其中一个目标是将每个粒子从最初嵌入冰中到最终密度贴图的所有相关元数据存档。第二个目标是开发一种能够合并密度图和相关元数据的档案数据格式。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Cryo-EM is a lengthy experimental and computational process, leading to a final 3-D map and related annotations. Each of the steps requires different expertise and skills. All of the investigators perform experiments somewhat differently in order to make each project work, and some manage to complete projects faster than others. In our Center, there are dozens of projects ongoing in any given month, each with different technical challenges and at different stages of development or progress. Some projects take only a few days to achieve a subnanometer resolution structure while others may take months or years to reach the same point. It is obvious that, in addition to various experimental and computational variables, investigators play a critical role in getting the project completed in a timely fashion. There are two aims in this core project. One aim is to archive all the metadata related to each particle from its initial embedding in ice to the final density map. The second aim is to develop an archival data format capable of amalgamating density maps and associated metadata.