ELECTRONIC NOTEBOOK FOR CRYO-EM

用于冷冻电镜的电子笔记本

• 批准号: 8168521
• 负责人: STEVEN J LUDTKE
• 金额: $ 8.6万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-15 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168521
• 关键词: 3-Dimensional; Archives; Computer Retrieval of Information on Scientific Projects Database; Development; Electronics; Funding; Grant; Ice; Institution; Maps; Metadata; Play; Process; Research; Research Personnel; Resolution; Resources; Role; Source; Staging; Structure; United States National Institutes of Health; Work; data format; density; particle; research study; skills

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cryo-EM is a lengthy experimental and computational process, leading to a final 3-D map and related annotations. Each of the steps requires different expertise and skills. All of the investigators perform experiments somewhat differently in order to make each project work, and some manage to complete projects faster than others. In our Center, there are dozens of projects ongoing in any given month, each with different technical challenges and at different stages of development or progress. Some projects take only a few days to achieve a subnanometer resolution structure while others may take months or years to reach the same point. It is obvious that, in addition to various experimental and computational variables, investigators play a critical role in getting the project completed in a timely fashion. There are two aims in this core project. One aim is to archive all the metadata related to each particle from its initial embedding in ice to the final density map. The second aim is to develop an archival data format capable of amalgamating density maps and associated metadata.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 冷冻电镜是一个漫长的实验和计算过程，最终形成最终的 3D 图和相关注释。每个步骤都需要不同的专业知识和技能。为了使每个项目发挥作用，所有研究人员进行的实验都略有不同，并且有些研究人员能够比其他研究人员更快地完成项目。在我们的中心，每个月都有数十个项目正在进行，每个项目都有不同的技术挑战，并且处于不同的开发或进展阶段。有些项目只需几天即可实现亚纳米分辨率结构，而其他项目可能需要数月或数年才能达到同一水平。显然，除了各种实验和计算变量之外，研究人员在项目及时完成方面也发挥着关键作用。这个核心项目有两个目标。一个目标是归档与每个粒子相关的所有元数据，从最初嵌入冰中到最终的密度图。第二个目标是开发一种能够合并密度图和相关元数据的档案数据格式。