SYNTHESIS OF PNA-ENCODED COMBINATORIAL PEPTIDE LIBRARIES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们建议使用多肽核酸(PNA)编码的组合多肽库作为工具来高通量筛选DNA结合蛋白的配体。为了在同一反应容器中合成PNA和氨基酸链，必须用不同的化学基团对它们进行保护。这鼓励了我们合成一种非商业用途的PNA，因为化学保护的氨基酸很容易获得。化学保护的PNA单体的多步骤合成将需要在每个反应步骤中对小分子产物进行评估。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We propose to use Peptide Nucleic Acid (PNA) -encoded combinatorial peptide libraries as a tool to perform high-throughput screening for ligands of DNA-binding proteins. In order to synthesize the PNA and amino acid chains in the same reaction vessel, they must be protected by different chemical groups. This has encouraged us to synthesize a non-commercially available PNA, since chemically protected amino acids are readily available. The multistep synthesis of chemically protected PNA monomers will require evaluation of the small molecule products at each reaction step.