SYNTHESIS OF PNA-ENCODED COMBINATORIAL PEPTIDE LIBRARIES

PNA 编码的组合肽库的合成

• 批准号: 7954648
• 负责人: ASEEM Z ANSARI
• 金额: --
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954648
• 关键词: Amino Acids; Computer Retrieval of Information on Scientific Projects Database; DNA-Binding Proteins; Evaluation; Funding; Grant; Institution; Ligands; Peptide Library; Peptide Nucleic Acids; Reaction; Research; Research Personnel; Resources; Source; United States National Institutes of Health; chemical group; combinatorial; high throughput screening; monomer; small molecule; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We propose to use Peptide Nucleic Acid (PNA) -encoded combinatorial peptide libraries as a tool to perform high-throughput screening for ligands of DNA-binding proteins. In order to synthesize the PNA and amino acid chains in the same reaction vessel, they must be protected by different chemical groups. This has encouraged us to synthesize a non-commercially available PNA, since chemically protected amino acids are readily available. The multistep synthesis of chemically protected PNA monomers will require evaluation of the small molecule products at each reaction step.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 我们建议使用肽核酸（PNA）编码的组合肽库作为对DNA结合蛋白配体进行高通量筛查的工具。 为了在同一反应容器中合成PNA和氨基酸链，必须由不同的化学基团保护。 这鼓励我们合成非商业上可用的PNA，因为很容易获得化学保护的氨基酸。 化学保护的PNA单体的多步合成将需要在每个反应步骤中评估小分子产物。