SYNTHESIS OF PNA-ENCODED COMBINATORIAL PEPTIDE LIBRARIES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 研究中心，而研究中心不一定是研究者所在的机构。 我们建议使用肽核酸（PNA）编码的组合肽库作为一种工具，进行高通量筛选的DNA结合蛋白的配体。 为了在同一反应容器中合成PNA和氨基酸链，它们必须被不同的化学基团保护。 这促使我们合成一种非商业可用的PNA，因为化学保护的氨基酸很容易获得。 化学保护的PNA单体的多步合成将需要在每个反应步骤评估小分子产物。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We propose to use Peptide Nucleic Acid (PNA) -encoded combinatorial peptide libraries as a tool to perform high-throughput screening for ligands of DNA-binding proteins. In order to synthesize the PNA and amino acid chains in the same reaction vessel, they must be protected by different chemical groups. This has encouraged us to synthesize a non-commercially available PNA, since chemically protected amino acids are readily available. The multistep synthesis of chemically protected PNA monomers will require evaluation of the small molecule products at each reaction step.