SYNTHESIS OF PNA-ENCODED COMBINATORIAL PEPTIDE LIBRARIES

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 我们建议使用肽核酸 (PNA) 编码的组合肽库作为工具来对 DNA 结合蛋白的配体进行高通量筛选。 为了在同一反应容器中合成PNA和氨基酸链，它们必须受到不同化学基团的保护。 这鼓励我们合成非商业可用的 PNA，因为化学保护的氨基酸很容易获得。 化学保护的 PNA 单体的多步合成需要对每个反应步骤的小分子产物进行评估。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We propose to use Peptide Nucleic Acid (PNA) -encoded combinatorial peptide libraries as a tool to perform high-throughput screening for ligands of DNA-binding proteins. In order to synthesize the PNA and amino acid chains in the same reaction vessel, they must be protected by different chemical groups. This has encouraged us to synthesize a non-commercially available PNA, since chemically protected amino acids are readily available. The multistep synthesis of chemically protected PNA monomers will require evaluation of the small molecule products at each reaction step.