GAL4/MED15 BINDING AND CONFORMATION STUDY

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 GAL基因构成了最具特征的真核转录调控系统之一，即酵母Saccharomyces gulea的半乳糖/melibioseregulon。酵母菌使用GAL酶将周围培养基中的半乳糖转化为葡萄糖-1-磷酸，其中两种是Gal 4和Gal 80。Gal 80在与Gal 4结合时充当转录阻遏物。我们的目标是通过NMR分析技术确定Gal 4和Gal 80结合位点的结构，结合前和结合后。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The GAL genes make up one of the best characterized eukaryotic systems of transcriptional regulation, the galactose/melibioseregulon of the yeast, Saccharomyces cerevisiea. Saccharomyces cerevisiea converts galactose from the surrounding medium toglucose-1-phosphate using the GAL enzymes, two of them being Gal4 and Gal80. Gal80 acts as a transcription repressor when bound to Gal4. Our goal is to determine the structure of the Gal4 and Gal80 binding sites, pre- and post-binding, via NMR analytical techniques.