ATFS, POLYAMIDE SYNTHESIS

ATFS，聚酰胺合成

• 批准号: 7954647
• 负责人: ASEEM Z ANSARI
• 金额: $ 0.01万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954647
• 关键词: Biochemical Pathway; Cells; Complement; Computer Retrieval of Information on Scientific Projects Database; Data; Engineering; Funding; Genes; Genetic; Genetic Transcription; Goals; Grant; High Pressure Liquid Chromatography; Institution; Methods; Nylons; Property; Proteins; Regulator Genes; Research; Research Personnel; Resolution; Resources; Small Interfering RNA; Source; Therapeutic Agents; United States National Institutes of Health; Validation; functional genomics; magnetic field; synthetic biology; tool; transcription factor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The major goals of our lab are to understand mechanisms that govern gene regulatory networks and to create synthetic molecules such as artificial transcription factors (ATFs) that can be engineered to control these networks in a predetermined manner. ATFs, due to their ability to stimulate transcription, will complement the siRNA approach and serve as powerful, functional, genomics tools that identify and control the gene networks which govern cell fate. They will also contribute to the nascent field of synthetic biology and metabolic network engineering by proving to be a versatile tool in the regulatation of submodules of gene networks. As the ultimate value of ATFs lies in their potential application as precision-tailored therapeutic agents, we aim to regulate transcriptional circuitry by creating synthetic molecules that mimic the properties of natural transcription factors or counteract the activity of malfunctioning genetic machinery. It is therefore of the utmost importance that the identities of the molecules (i.e., synthetic mimics) we synthesize are confirmed via thorough structural analyses before proceeding further. We wish to incorporate NMR data as the primary method of structural validation, corroborated by mass spec and HPLC data. Although this particular project probably requires only low field NMR analysis (400 MHz), we are currently planning other projects which would require resolution of more complicated proteins therefore necessitating the use of much higher magnetic fields.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 我们实验室的主要目标是了解控制基因调节网络并创建合成分子（例如人工转录因子（ATF））的机制，可以设计以预定的方式控制这些网络。 ATF由于其刺激转录的能力，将补充siRNA方法，并充当强大，功能性的基因组学工具，可识别和控制控制细胞命运的基因网络。 它们还将通过证明是基因网络子模块的多功能工具，为合成生物学和代谢网络工程的新生领域做出贡献。由于ATF的最终值在于它们作为精确限制治疗剂的潜在应用，因此我们旨在通过创建模仿自然转录因子的特性或抵消遗传机械功能的合成分子来调节转录电路。因此，最重要的是，我们合成的分子的身份（即合成模拟物）在进一步进行之前通过彻底的结构分析得到证实。 我们希望将NMR数据纳入结构验证的主要方法，并通过质量规格和HPLC数据证实。 尽管这个特定的项目可能只需要低场NMR分析（400 MHz），但我们目前正在计划其他项目，这些项目需要解决更复杂的蛋白质，因此需要使用更高的磁场。