GAL4/MED15 BINDING AND CONFORMATION STUDY

GAL4/MED15 结合和构象研究

• 批准号: 8168964
• 负责人: ASEEM Z ANSARI
• 金额: $ 1.62万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168964
• 关键词: Binding; Binding Sites; Computer Retrieval of Information on Scientific Projects Database; Enzymes; Funding; Galactose; Genes; Goals; Grant; Institution; Molecular Conformation; Research; Research Personnel; Resources; Saccharomyces; Source; Structure; System; Techniques; Transcription Repressor/Corepressor; Transcriptional Regulation; United States National Institutes of Health; Yeasts; inorganic phosphate

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The GAL genes make up one of the best characterized eukaryotic systems of transcriptional regulation, the galactose/melibioseregulon of the yeast, Saccharomyces cerevisiea. Saccharomyces cerevisiea converts galactose from the surrounding medium toglucose-1-phosphate using the GAL enzymes, two of them being Gal4 and Gal80. Gal80 acts as a transcription repressor when bound to Gal4. Our goal is to determine the structure of the Gal4 and Gal80 binding sites, pre- and post-binding, via NMR analytical techniques.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 Gal基因构成了真核转录调控系统中最具特色的系统之一，即酿酒酵母的半乳糖/半乳糖生物调控系统。酿酒酵母使用GAL酶将半乳糖从周围的介质中转化为葡萄糖-1-磷酸，其中两种是Gal4和Gal80。Gal80与Gal4结合时可作为转录抑制因子。我们的目标是通过核磁共振分析技术确定Gal4和Gal80结合位点的结构，结合前和结合后。