STRUCTURE AND DNA BINDING STUDIES OF NANOG, SOX2, AND OCT4

NANOG、SOX2 和 OCT4 的结构和 DNA 结合研究

• 批准号: 8361239
• 负责人: ASEEM Z ANSARI
• 金额: $ 0.8万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361239
• 关键词: Binding; DNA Binding; Elements; Embryo; Endoderm; Funding; Gene Targeting; Grant; Growth Factor; Human; Mus; National Center for Research Resources; Pathway interactions; Phenotype; Principal Investigator; Research; Research Infrastructure; Resources; Roentgen Rays; Source; Structure; Transcriptional Regulation; United States National Institutes of Health; cost; embryonic stem cell; homeodomain; pluripotency; promoter; stem; stem cell biology; transcription factor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. NANOG, Sox2 and Oct4 are transcription factors with key functions in mammalian stem cell biology. They are central of pluripotency in ES cells. Loss of NANOG induces differentiation towards an endoderm-like lineage in embryonic stem (ES) cells, whereas forced expression retains the ES phenotype in the absence of the otherwise essential growth factors. NANOG binds to promoter elements of hundreds of target genes and regulates their expression. The mechanism of this transcriptional regulation is not yet known. Currently, one X-ray crystal structure of the homeodomain of murine Nanog is available in the PDB; however, numerous studies have shown that the pathways maintaining pluripotency are different between human and mouse ES cells. We plan to determine the structure and DNA binding studies of NANOG, Sox2 and Oct4 by NMR.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 Nanog，Sox2和Oct4是具有哺乳动物干细胞生物学关键功能的转录因子。它们是ES细胞多能性的中心。 Nanog的丧失诱导了胚胎（ES）细胞中内胚层样谱系的分化，而强迫表达在没有原本必要的生长因子的情况下保留了ES表型。纳米与数百个靶基因的启动子元素结合并调节其表达。该转录调控的机制尚不清楚。当前，PDB中有一个鼠Nanog同源域的X射线晶体结构。然而，许多研究表明，人类和小鼠ES细胞之间维持多能性的途径不同。我们计划通过NMR确定Nanog，Sox2和Oct4的结构和DNA结合研究。