ATFS, POLYAMIDE SYNTHESIS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们实验室的主要目标是了解控制基因调控网络的机制，并创建合成分子，如人工转录因子(ATF)，这些分子可以被设计成以预定的方式控制这些网络。ATF由于其刺激转录的能力，将补充siRNA方法，并作为强大的、功能强大的基因组学工具，识别和控制支配细胞命运的基因网络。它们还将被证明是基因网络子模块调控的通用工具，从而为合成生物学和新陈代谢网络工程这一新兴领域做出贡献。由于ATF的最终价值在于它们作为精确定制的治疗剂的潜在应用，我们的目标是通过创建模拟自然转录因子的属性或中和故障遗传机械的活动的合成分子来调节转录电路。因此，在进一步进行之前，通过彻底的结构分析来确认我们合成的分子(即合成的仿制品)的身份是至关重要的。我们希望将核磁共振数据作为结构验证的主要方法，并得到质谱分析和高效液相数据的证实。虽然这个特殊的项目可能只需要低场核磁共振分析(400 MHz)，但我们目前正在计划其他项目，这些项目将需要解析更复杂的蛋白质，因此需要使用更高的磁场。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The major goals of our lab are to understand mechanisms that govern gene regulatory networks and to create synthetic molecules such as artificial transcription factors (ATFs) that can be engineered to control these networks in a predetermined manner. ATFs, due to their ability to stimulate transcription, will complement the siRNA approach and serve as powerful, functional, genomics tools that identify and control the gene networks which govern cell fate. They will also contribute to the nascent field of synthetic biology and metabolic network engineering by proving to be a versatile tool in the regulatation of submodules of gene networks. As the ultimate value of ATFs lies in their potential application as precision-tailored therapeutic agents, we aim to regulate transcriptional circuitry by creating synthetic molecules that mimic the properties of natural transcription factors or counteract the activity of malfunctioning genetic machinery. It is therefore of the utmost importance that the identities of the molecules (i.e., synthetic mimics) we synthesize are confirmed via thorough structural analyses before proceeding further. We wish to incorporate NMR data as the primary method of structural validation, corroborated by mass spec and HPLC data. Although this particular project probably requires only low field NMR analysis (400 MHz), we are currently planning other projects which would require resolution of more complicated proteins therefore necessitating the use of much higher magnetic fields.