ATFS, POLYAMIDE SYNTHESIS

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 我们实验室的主要目标是了解控制基因调控网络的机制，并创建合成分子，如人工转录因子（ATFs），这些分子可以被设计成以预定的方式控制这些网络。ATFs由于其刺激转录的能力，将补充siRNA方法，并作为鉴定和控制控制细胞命运的基因网络的强大的功能性基因组学工具。 它们还将有助于合成生物学和代谢网络工程的新生领域，证明是一个多功能的工具，在调节基因网络的子模块。由于ATF的最终价值在于其作为精确定制的治疗剂的潜在应用，我们的目标是通过创建模拟天然转录因子的特性或抵消故障遗传机制的活性的合成分子来调节转录电路。因此，最重要的是分子的身份（即，合成模拟物）在进一步进行之前通过彻底的结构分析来确认。 我们希望将NMR数据作为结构验证的主要方法，并通过质谱和HPLC数据进行确证。 虽然这个特定的项目可能只需要低场NMR分析（400 MHz），但我们目前正在计划其他项目，这些项目需要解析更复杂的蛋白质，因此需要使用更高的磁场。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The major goals of our lab are to understand mechanisms that govern gene regulatory networks and to create synthetic molecules such as artificial transcription factors (ATFs) that can be engineered to control these networks in a predetermined manner. ATFs, due to their ability to stimulate transcription, will complement the siRNA approach and serve as powerful, functional, genomics tools that identify and control the gene networks which govern cell fate. They will also contribute to the nascent field of synthetic biology and metabolic network engineering by proving to be a versatile tool in the regulatation of submodules of gene networks. As the ultimate value of ATFs lies in their potential application as precision-tailored therapeutic agents, we aim to regulate transcriptional circuitry by creating synthetic molecules that mimic the properties of natural transcription factors or counteract the activity of malfunctioning genetic machinery. It is therefore of the utmost importance that the identities of the molecules (i.e., synthetic mimics) we synthesize are confirmed via thorough structural analyses before proceeding further. We wish to incorporate NMR data as the primary method of structural validation, corroborated by mass spec and HPLC data. Although this particular project probably requires only low field NMR analysis (400 MHz), we are currently planning other projects which would require resolution of more complicated proteins therefore necessitating the use of much higher magnetic fields.