HIGH QUALITY CATALOG OF PROTEOTYPIC PEPTIDES FROM HUMAN HEART

人类心脏蛋白肽的高质量目录

• 批准号: 7957751
• 负责人: Christine Wu Nordahl
• 金额: $ 2.77万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957751
• 关键词: Biological Assay; Biological Markers; Biology; Cardiac; Cataloging; Catalogs; Computer Retrieval of Information on Scientific Projects Database; Data Set; Development; Frequencies; Funding; Fungal Genome; Future; Grant; Heart; Heart Diseases; Human; Institution; Knowledge; Left; Mass Spectrum Analysis; Monitor; Peptides; Protein Analysis; Proteins; Proteomics; Proxy; Research; Research Personnel; Resources; Shotguns; Source; Technology; Tissue Sample; Tissues; United States National Institutes of Health; Ventricular; base; research study; trizol

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Proteomics research is beginning to expand beyond the more traditional shotgun analysis of protein mixtures to include targeted analyses of specific proteins using mass spectrometry. Integral to the development of a robust assay based on targeted mass spectrometry is prior knowledge of which peptides provide an accurate and sensitive proxy of the originating gene product (i.e. proteotypic peptides). To develop a catalog of proteotypic peptides in human heart, TRIzol? extracts of left-ventricular tissue from non-failing and failing human heart explants were optimized for shotgun proteomic analysis using Multi-dimensional Protein Identification Technology (MudPIT). Ten replicate MudPIT analyses were performed on each tissue sample and resulted in the identification 30,605 unique peptides with a q-value of 0.01 or better, corresponding to 7,138 unique human heart proteins. Experimental observation frequencies were assessed and used to select over 4,476 proteotypic peptides for 2,558 heart proteins. This human cardiac dataset can serve as a public reference to guide the selection of proteotypic peptides for future targeted mass spectrometry experiments monitoring potential protein biomarkers of human heart diseases.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 蛋白质组学研究开始扩展超出对蛋白质混合物的更传统的shot弹枪分析，包括使用质谱法对特定蛋白质进行靶向分析。 基于靶向质谱的强大测定法的发展不可或缺的是，先验知识的肽提供了原始基因产物（即蛋白质型肽）的准确而敏感的代理。 在人心脏中开发蛋白质肽的目录，trizol？使用多维蛋白质鉴定技术（MUDPIT）优化了从非失败和人类心脏外植体中左心室组织的提取物进行shot弹枪蛋白质组学分析。 在每个组织样品上进行了十个重复的泥盘分析，并导致识别30,605 Q值为0.01或更高的独特肽，对应于7,138个独特的人心脏蛋白。 评估实验观察频率，并用于选择超过4,476种蛋白质肽的2,558个心脏蛋白。 该人类心脏数据集可以作为公众参考，以指导蛋白质肽的选择，以监测人类心脏病的潜在蛋白质生物标志物的未来靶向质谱实验。