PROTEOMIC PROFILING OF INTEGRAL MEMBRANE PROTEIN TOPOLOGY

整体膜蛋白拓扑结构的蛋白质组学分析

• 批准号: 8365856
• 负责人: Christine Wu Nordahl
• 金额: $ 0.65万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365856
• 关键词: Biochemical; Biology; Digestion; Funding; Fungal Genome; Goals; Grant; Integral Membrane Protein; Lipid Bilayers; Membrane Proteins; National Center for Research Resources; Peptides; Principal Investigator; Proteomics; Research; Research Infrastructure; Resources; Source; United States National Institutes of Health; cost; improved

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The goal of the project is to use proteolytic digestion combined with the characterization of the resulting peptides to improve our understanding of membrane protein topology. Biochemical approaches are being applied to characterize both the soluble "loops" and the lipid bilayer embedded peptides.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 该项目的目标是使用蛋白水解酶结合所得多肽的表征来提高我们对膜蛋白拓扑的理解。生物化学方法正被应用于可溶“环”和脂双层包埋肽的表征。