OPERATOR TRAINING IN BIOLOGICAL MASS SPECTROMETRY

生物质谱操作员培训

• 批准号: 7955901
• 负责人: Catherine E. Costello
• 金额: $ 0.59万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955901
• 关键词: Amyloid; Amyloid Proteins; Amyloidosis; Biological; Biology; Complex; Computer Retrieval of Information on Scientific Projects Database; Data; Fatty acid glycerol esters; Funding; Gel; Grant; Hospitals; Human; Human Resources; Institution; Ions; Italy; Korea; Laboratories; Learning; Mass Spectrum Analysis; Medicine; Patients; Postdoctoral Fellow; Process; Protein Analysis; Reflex action; Research; Research Personnel; Resources; Sampling; Scientist; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Staging; System; Training; United States National Institutes of Health; Universities; Visit; career; graduate student; instrument; instrumentation; liquid chromatography mass spectrometry

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Training on laboratory instruments is provided for investigators at various stages of their professional careers, in addition to the resident postdoctoral fellows and sabbatical guests. R. Theberge is responsible for training on the Bruker Reflex IV MALDI reflectron TOF MS. M. J. C. Miller has been responsible for the Bruker Esquire ion trap, R. Seward for the Q-Star qoTOF MS instruments and U. Sommer, for the ESI Quattro II triple quadrupole LC/MS system. Under the direction of these senior personnel, new postdoctoral fellows, visiting scientists and graduate students have learned to operate the introductory level instruments, have moved upward to the more complex instrumentation and and have been instructed how to process and interpret the resulting data. During this year Ms. Eunjung Song, a graduate student at Seoul National University, Korea, visited for six months and has retured to Seoul. Francesca Lavatelli, MD, from the Univ. of Pavia, Italy, made rapid progress with 2D gel analysis of amyloid proteins and others components found in human fat samples and learned to interpret the MS and MS/MS data. After she returned to Italy in January 2007, she took on responsibility for amyloid protein analysis in samples obtained from familial amyloid patients and other with sporadic or undiagnosed amyloid diseases at the hospital and research center in Pavia where she is employed.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 除了常驻博士后研究员和休假客人外，还为处于职业生涯各个阶段的研究人员提供实验室仪器培训。R. Theberge负责Bruker Reflex IV MALDI反射TOF MS的培训。J. C.米勒一直负责布鲁克Esquire离子阱，R。Seward的Q-Star qoTOF MS仪器和U。Sommer，用于ESI Quattro II三重四极杆LC/MS系统。在这些高级人员的指导下，新的博士后研究员，访问科学家和研究生学会了操作入门级仪器，已经上升到更复杂的仪器，并已被指示如何处理和解释所产生的数据。今年，韩国首尔国立大学的研究生Eunjung Song女士访问了六个月，并返回首尔。来自意大利帕维亚大学的Francesca Lavatelli医学博士在人体脂肪样本中发现的淀粉样蛋白和其他成分的2D凝胶分析方面取得了快速进展，并学会了解读MS和MS/MS数据。2007年1月她回到意大利后，在她工作的帕维亚医院和研究中心负责从家族性淀粉样蛋白患者和其他散发性或未确诊的淀粉样蛋白疾病患者中获得的样本的淀粉样蛋白分析。