STRUCTURE FUNCTION RELATIONSHIPS IN POLYSACCHARIDES

多糖的结构功能关系

• 批准号: 7956845
• 负责人: SRINIVAS JANASWAMY
• 金额: $ 0.47万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7956845
• 关键词: Behavior; Biopolymers; Complex; Computer Retrieval of Information on Scientific Projects Database; Fiber; Food; Funding; Glycosaminoglycans; Goals; Grant; Institution; Lateral; Least-Squares Analysis; Link; Methods; Molecular Models; Molecular Structure; Pattern; Pharmacologic Substance; Plants; Polymers; Polysaccharides; Process; Research; Research Personnel; Resources; Series; Solutions; Solvents; Source; Specimen; Stretching; Structure; Structure-Activity Relationship; System; Temperature; United States National Institutes of Health; Wood material; analog; aqueous; galactoglucomannan; gellan; glucuronoxylomannan; lambda Carrageenan; molecular modeling; programs; solute; structural biology; three dimensional structure; xanthan gum

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our long term goals are to elucidate the molecular structures of (non-crystalline) biopolymers, especially polysaccharides, and their interactions with solvent and solute molecules and the synergistic interactions in mixed polysaccharides towards an in depth understanding of their functional behavior. In this regard, the main thrust of the present proposal is centered on determining the three-dimensional structures of a series of biologically important and industrially useful polysaccharides. In this reagard, our ongoing study will include, but not limited to (as need arises), the specimens from algal (iota, kappa and lambda carrageenans); bacterial (gellan analog and cepacian); fungal (glucuronoxylomannan); plant (rhamnogalacturonan); wood (galactoglucomannan) and a binary system (bacterial xanthan:plant glucomannan and bacterial acetan:plant glucomannan). These polysaccharides or polysaccharide complexes have an inherent tendency to form helical structures with only limited lateral ordering and are not amenable for growing single crystals. Hence, fiber diffraction is the only method of choice for visualizing their three-dimensional organization. Such structural information is essential towards understanding their interactions with solvent and solute molecules as well as with other polysaccharides for their effective utilization in food and pharmaceutical applications. The results will further highlight the conformational space available to polysaccharides as they occur in glycosaminoglycans and cellular walls. Oriented and polycrystalline fibers will be obtained from aqueous polymer solutions through stretching process at room temperature. Linked-Atom Least-Squares (LALS) program will be used to build the molecular models to be in conformity with the diffraction pattern and to solve the structure.

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