ANALYTICAL TECHNIQUES FOR STRUCTURE ANALYSIS OF GLYCOSAMINOGLYCANS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 这是一门新课程，于2008年8月25日至27日首次授课。参加者学习了糖胺聚糖结构表征的基本技术，其中包括使用适当的酶将糖胺聚糖多糖解聚成其二糖成分。双糖经离子交换层析分离，紫外检测，2-氰基乙酰胺衍生，荧光检测。在单独的实验中，通过亚硝酸水解糖胺聚糖并分析产物。还包括用于分析糖胺聚糖衍生的二糖和寡糖的质谱技术。具有基本生物化学技术的经验是参加的先决条件。 共有8名参与者，其中7名来自非营利组织，1名来自工业界。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This was a new course, which was taught for the first time August 25-27, 2008. Participants learnt basic techniques for the structural characterization of glycosaminoglycans (GAGs), which included depolymerization of the GAG polysaccharides to its disaccharide constituents using appropriate enzymes. The disaccharides were separated by ion exchange chromatography and the products detected by U.V. The disaccharides were derivatized with 2-cyanoacetamide and were detected with fluorescent detection. In a separate experiment, the glycosaminoglycans were hydrolyzed by nitrous acid and the products analyzed. Mass spectrometry techniques for analysis of di- and oligosaccharides derived from glycosaminoglycans were also covered. Experience with basic biochemical techniques is a prerequisite for participation. A total of 8 participants with 7 from non-profit and 1 from industry.