COMPARATIVE PROTEOMICS AND GLYCOPROTEOMICS OF PROTEINS SECRETED FROM B CINEREA

灰霉病菌分泌蛋白质的比较蛋白质组学和糖蛋白质组学

• 批准号: 7955999
• 负责人: RON ORLANDO
• 金额: $ 4.44万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955999
• 关键词: Affect; Botrytis; Cell Wall; Computer Retrieval of Information on Scientific Projects Database; Development; Economics; Enzymes; Flowers; Funding; Grant; Gray unit of radiation dose; Growth; Harvest; Infection; Institution; Molds; Phase; Plants; Prevention strategy; Process; Proteins; Proteome; Proteomics; Recombinant Proteins; Research; Research Personnel; Resources; Source; Technology; Tissues; Trees; United States National Institutes of Health; comparative; fruits and vegetables; fungus; glycosylation; pathogen; protein structure

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The fungus Botrytis cinerea is a widespread plant pathogen that affects many vegetables and fruits, as well as a large number of shrubs, trees, flowers, and weeds by causing grey mold or soft rot on the host tissues. B. cinerea mainly infects dicotyledons and non-graminaceous monocotyledons, and the infection not only occurs during the growth phase of crops but also during storage of the harvested products. Thus, it is responsible for significant economic losses. A great deal of research has focused on understanding the B. cinerea infection process, which in turn directs the development of effective prevention strategies. B. cinerea must breach the cell wall during the infection process and accordingly secretes a great number of enzymes that can degrade cell walls. Little is known concerning the identity of these enzymes. The majority of these cell wall degrading enzymes are glycosylated. Although the glycosylation on several recombinant proteins have been characterized, very little is known on the native enzymes, despite the importance of glycosylation on protein structure and activity. To this end, we have started to characterize the secreted proteome and glycoproteome of this fungus.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 真菌辣椒粉是一种广泛的植物病原体，影响了许多蔬菜和水果，以及 通过在宿主组织上引起灰色模具或软腐烂，大量的灌木，树木，花和杂草。 B. cinerea 主要是感染二元子和非同性恋单子叶植物，并且感染不仅发生在此期间 农作物的生长阶段，也是在收获产品的储存过程中。因此，它是重要的 经济损失。大量的研究集中在理解B. cinerea感染过程中，这在 转弯指导有效的预防策略的制定。 B.灰纳瑞亚必须在 感染过程，因此分泌大量会降解细胞壁的酶。鲜为人知 关于这些酶的身份。这些细胞壁降解酶的大多数是糖基化的。 尽管已经表征了几种重组蛋白上的糖基化，但在 尽管糖基化对蛋白质结构和活性的重要性，但天然酶。为此，我们有 开始表征这种真菌的分泌蛋白质组和糖蛋白酶。