DEVELOPMENT OF TECHNIQUES FOR QUANTITATIVE GLYCOMICS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 多糖表达的定量比较需要高度灵敏的方法来区分和识别 具有微妙不同结构的单个多聚糖。质谱仪(MS)满足了这些要求，同时提供了 快速可靠的分析复杂混合物的方法，因此在血糖研究中得到了广泛的应用。一 使用MS对生物分子进行定量的限制是不同分子物种的电离效率可以 根据分析物的分子质量、质子/阳离子亲和力、表面活性、 存在与分析物的电离竞争或干扰的化合物等。我们参与了 设计新的方法来提高比较血糖实验的准确性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The quantitative comparison of glycan expression requires highly sensitive methods that can distinguish and identify individual glycans with subtly different structures. Mass spectrometry (MS) fulfills these requirements while providing a rapid and reliable method to analyze complex mixtures, and therefore has been widely used in glycomic studies. One limitation of using MS for the quantitation of biomolecules is that the ionization efficiencies of distinct molecular species can differ significantly, depending on such factors as the analyte's molecular mass, proton/cation affinity, surface activity, the presence of compounds which compete with or interfere with the ionization of the analyte, etc. We are involved in devising new methodologies to improve the accuracy of comparative glycomic experiments.